| 摘要: |
| 【目的】研究感染绿脓杆菌（Pseudomonas aeruginosa）和金黄色葡萄球菌（Staphyloccocus aureus）后，家蚕中肠和脂肪体中6种抗菌肽基因表达的变化，为家蚕肠道免疫研究提供一定的理论依据。【方法】给家蚕喂食感染绿脓杆菌和金黄色葡萄球菌，以喂生理盐水为对照，利用半定量RT-PCR方法，检测感染不同时间（1，2，4，8，16，24 h）后中肠和脂肪体中6种抗菌肽基因表达的变化。【结果】喂食绿脓杆菌1，2 h后，中肠中抗菌肽Gloverin2、Lebocin、CecropinB6、CecropinD和Moricin的相对表达量显著上调；喂食金黄色葡萄球菌1，2，4 h后，中肠中6种抗菌肽的相对表达量均显著上调。在脂肪体中，感染两种细菌后，仅抗菌肽CecropinD、Moricin和Attacin2相对表达量的变化较为显著。【结论】家蚕在感染不同细菌后所诱导的免疫机制不同。细菌经由口器进入家蚕肠道，可同时引起上皮免疫反应和体液免疫反应。 |
| 关键词: 家蚕 细菌 抗菌肽基因 半定量RT-PCR |
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| 基金项目:国家重大基础研究发展计划（973计划）子课题（2012CB114604）；教育部基本科研业务费专项（CX200908） |
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| Expression of 6 antimicrobial peptide genes in silkworm,Bombyx mori after oral bacterial infection |
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WANG Yan-wen,LÜ Zhi-qiang
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| Abstract: |
| 【Objective】The expression of 6 antimicrobial peptide genes after oral infection with Pseudomonas aeruginosa and Staphylococcus aureus was studied to provide theoretical basis for gut immune of silkworm.【Method】Using physiological saline as control check,the expression of 6 antimicrobial peptide genes in midgut and fat body of silkworm after 1,2,4,8,16,and 24 h Pseudomonas aeruginosa and Staphylococcus aureus infection was studied by semi-quantitative RT-PCR.【Result】Silkworm significantly up-regulated the relative expression of Gloverin2,Lebocin,CecropinB6,CecropinD and Moricin after infection 1,2 h with P.aeruginosa and the relative expression of all 6 antimicrobial peptides increased after infection 1,2,and 4 h with S.aureus in midgut.After oral infection with two bacteria,the relative expression of CecropinD,Moricin and Attacin2 was significant different in fat body.【Conclusion】The immune mechanisms of silkworm against different bacteria were different.Bacteria in intestine via mouthparts could induce both epithelial immune response and humoral immune response. |
| Key words: silkworm bacteria antibacterial peptides gene semi-quantitative RT-PCR |
