| 摘要: |
| 【目的】研究茶条槭叶总酚的提取工艺，并对茶条槭叶总酚提取物的抗氧化活性进行评价，为茶条槭叶的开发利用提供依据。【方法】以乙醇为溶剂, 以总酚提取率为考察指标，采用超声辅助提取法处理茶条槭叶，先通过单因素试验确定乙醇体积分数、超声提取时间和液固比的适宜范围，再应用中心组合设计和响应面分析相结合的方法筛选最佳提取条件；最后采用铁离子还原法、DPPH自由基清除法测定茶条槭叶提取物的抗氧化能力。【结果】超声波辅助提取茶条槭叶总酚的最佳工艺条件为：乙醇体积分数55%，超声提取时间46 min，液固比246 mL/g，在此条件下茶条槭叶总酚提取率为 (10.95±0.22)%。茶条槭叶总酚提取液对铁离子还原的IC50为 (0.157±0.005) mg/mL，对DPPH自由基的IC50为 (0.073±0.003) mg/mL。【结论】超声提取法可以避免总酚在较高温度下的分解，且优化后提取率较高，可以用于茶条槭叶总酚的提取；茶条槭叶提取液具有较高的抗氧化活性。 |
| 关键词: 茶条槭叶 总酚 超声提取 中心组合设计 响应面分析 抗氧化活性 |
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| 基金项目:国家科技支撑计划项目（2011BAI03B01）；国家公益性行业科研专项（201303111）；吉林省科技发展计划项目（20126046,YYZX201258,20140204013YY）；国家自然科学基金项目（31000154） |
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| Optimization of total phenol extraction from leaves of Acer ginnala and its antioxidant activity |
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ZHAO Yan,YU Ting,CAI En-bo,et al
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| Abstract: |
| 【Objective】This paper investigated the extraction process of total phenol from Acer ginnala leaves and evaluated its antioxidant activity to provide basis for the development and utilization of A.ginnala leaves.【Method】A.ginnala leaves were processed using ultrasonic assisted method with ethanol as the solvent and total phenol extraction yield as examining index.First,ethanol volume fraction,suitable range of ultrasonic extraction time,and solid-liquid ratio were determined by single factor experiments.Then,central composite design and response surface analysis method were applied for screening the best extraction conditions.At last,the iron reduction method and DPPH free radical scavenging assay were adopted to determine antioxidant capacity of A.ginnala leaves extract.【Result】The optimum conditions for ultrasonic assisted extraction were ethanol volume fraction 55%,extraction time 46 min,and liquid-solid ratio 246 mL/g with the optimal total phenol yield of (10.95±0.22)%.The iron ions reduction IC50 of extract was (0.157±0.005) mg/mL and that for DPPH was (0.073±0.003) mg/mL.【Conclusion】Ultrasonic extraction method can avoid the decomposition of total phenol under high temperature,and had high yield after optimization.Thus,it can be used for extraction of total phenol from A.ginnala leaves,which had high antioxidant activity. |
| Key words: leaves of Acer ginnala total phenolic ultrasonic extraction central composite design response surface analysis antioxidant activity |
