| 摘要: |
| 【目的】克隆猪核受体4A1 (Nuclear receptor subfamily 4,group A,member 1,NR4A1) DNA序列全长，并对CDS序列及其表达模式进行分析。【方法】以未成年长大母猪的卵巢为材料，采用电子克隆技术，对猪NR4A1 DNA序列进行分离和测序，并对猪NR4A1基因序列进行生物信息学分析；利用性腺激素（人绒毛膜促性腺激素（Human chorionic gonadotropin，HCG）和孕马血清促性腺激素（Pregnant mare serum gonadotropin，PMSG））处理卵巢颗粒细胞,采用RT-PCR方法分析猪NR4A1基因的表达模式。【结果】获得猪NR4A1基因4 870 bp 的DNA序列,其中CDS全长序列为1 734 bp，共编码572个氨基酸；猪NR4A1基因编码的氨基酸序列与人的对应氨基酸序列的相似性最高(97%)，其次为小鼠(94%)；Expasy软件预测结果表明，NR4A1蛋白的氨基酸序列非常保守，其中包含2个锌指特征（NR C4-type），一个是激素受体超家族，另一个是Zf-C4超家族；猪卵巢颗粒细胞在性腺激素处理后，可诱导猪NR4A1基因在卵泡发育和排卵时期瞬时表达。【结论】猪NR4A1基因的CDS区在物种间保守性较强，推测猪NR4A1基因调节卵泡的发育和排卵过程。 |
| 关键词: 猪 NR4A1 序列分析 表达模式 |
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| 基金项目:安徽省农业科学院科技创新团队项目（13C0405）；安徽省农业科学院院长青年基金项目（14B0427） |
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| Cloning,sequence analysis and expression pattern of porcine NR4A1 gene |
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LIU Lin-qing,LI Feng-e,XIONG Yuan-zhu,et al
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| Abstract: |
| 【Objective】The genomic sequence of porcine NR4A1 gene was cloned and the temporal and spatial expression patterns were studied.【Method】Using ovary of minor sow as material,the structure of the porcine NR4A1 gene was analyzed by bioinformatics methods.The expression patterns of NR4A1 gene were analyzed by RT-PCR after ovarian granulosa cells were treated with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG).【Result】The genomic sequence with 4 870 bp of NR4A1 gene was obtained,which contained 1 734 bp CDS and encoded 572 amino acids.The amino acid sequences exhibited the greatest identity with human (97%),followed by house mouse (94%).Expasy software estimation showed that NR4A1 protein was conservative including 2 NR C4-type,one was hormone receptor superfamily and the other was Zf-C4 superfamily.The porcine NR4A1 gene had a rapid expression pattern after granulose cells were treated with PMSG/HCG.【Conclusion】NR4A1 gene was highly conservative among species and it involved in the regulation of follicular development,ovulation and e luteinizing process. |
| Key words: pig NR4A1 sequence analysis expression pattern |
