| 摘要: |
| 【目的】研究β-伴大豆球蛋白(β-Conglycinin)对鲤幼鱼、稚鱼蛋白酶和淀粉酶活力的影响。【方法】以初始体质量为(10.06±0.14) g/尾的鲤稚鱼和(110.23±0.23) g/尾的鲤幼鱼为研究对象，以鱼粉为动物蛋白源，面粉、糊精为糖源，混合油脂(m(鱼油)∶m(玉米油)=1∶1)为脂肪源，分别配制5种等氮（鲤幼鱼和稚鱼粗蛋白质量分数分别为36%和40%）、等能(鲤幼鱼和稚鱼总能分别是15.2和16.9 MJ/kg) 的半精制饲料，其β-Conglycinin的添加量(质量分数)分别为0(CK)，2.0%，4.0%，6.0%和8.0%，每组饲料设3个重复，在控温单循环养殖系统中进行为期8周的饲养试验，试验结束后，取鲤幼鱼、稚鱼的前、中、后肠道和肝胰脏，分别用福林 酚试剂法和淀粉酶试剂盒法，测定肠道和肝胰脏蛋白酶及淀粉酶的活力。【结果】鲤幼鱼肝胰脏蛋白酶活力各组之间差异不显著(P＞0.05)；β-Conglycinin添加量为6.0%和8.0%组的前肠、中肠蛋白酶活力显著低于对照组(P＜0.05)；而β-Conglycinin添加量为8.0%组后肠蛋白酶活力显著低于对照组(P＜0.05)。在鲤稚鱼肝胰脏和后肠，β-Conglycinin添加量为8.0%组的蛋白酶活力显著低于对照组(P＜0.05)；前肠蛋白酶活力则以2.0%，4.0%，6.0%和8.0%添加组显著低于对照组(P＜0.05)；中肠蛋白酶活力为4.0%，6.0%和8.0%添加组显著低于对照组(P＜0.05)。β-Conglycinin对鲤幼鱼和稚鱼肝胰脏、前肠、中肠及后肠淀粉酶活力均无显著影响(P＞0.05)。【结论】鲤幼鱼配合饲料中β-Conglycinin的添加量不应超过6.0%；鲤稚鱼配合饲料中β-Conglycinin的添加量不应超过2.0%。 |
| 关键词: β-伴大豆球蛋白 鲤 幼鱼 稚鱼 蛋白酶 淀粉酶 |
| DOI： |
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| 基金项目:吉林省教育厅项目（2012043） |
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| Effects of β-Conglycinin on activities of protease and amylase in juvenile and larval common carps |
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XING Xiu-ping,LAI Hong-e,ZHAO Han,et al
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| Abstract: |
| 【Objective】The research investigated the effects of β-Conglycinin on activities of protease and amylase in juvenile and larval common carps.【Method】Larval and juvenile common carps with the initial weights of (10.06±0.14) g/tail and (110.23±0.23) g/tail were used as experimental objects for eight week feeding trial at controlled temperature in single recirculating system.Five diets with identical nitrogen (total crude protein contents for juvenile and larval were 36% and 40%,respectively) and energy (total energies for juvenile and larval were 15.2 and 16.9 MJ/kg,respectively) as well as different β-Conglycinin contents (0(CK),2.0%,4.0%,6.0%,and 8.0%) were provided.Fish meal was animal protein source,dextrin and flour were carbohydrate source,and mixed oil (m(corn oil)∶m(fish oil)=1∶1) was fat source.Each group had three repetitions.The activities of protease and amylase in foregut,midgut and hepatopancreas were detected using Folin-phenol method and Amylase kit.【Result】No significant effects of β-Conglycinin on activities of protease in hepatopancreas of juvenile common carps were observed (P＞0.05).The activities of protease in foregut and midgut of juvenile common carps in 6.0% and 8.0% groups were significantly lower than that of the control group (P＜0.05),and that in hindgut of 8.0% group were significantly lower than that of the control group (P＜0.05).The activities of protease in hepatopancreas and hindgut of larval common carps in 8.0% groups were significantly lower than that of the control group (P＜0.05),that of 2.0%,4.0%,6.0%,and 8.0% groups were significantly lower (P＜0.05) in foregut,and that of 4.0%,6.0%,and 8.0% groups were significantly lower (P＜0.05) in midgut.In addition,no significant effects of β-Conglycinin on activities of amylase in hepatopancreas and tract of both juvenile and larval common carps were observed (P＞0.05).【Conclusion】The amount of β-Conglycinin should be less than 6.0% and 2.0% in the diet of juvenile and larval common carp,respectively. |
| Key words: β-Conglycinin Cyprinus carpio juvenile common carp larval common carp protease amylase |
