| 摘要: |
| 【目的】探索绵羊BMPR-IB基因第7外显子多态性与产羔数的相关性，寻找控制绵羊繁殖力的分子标记位点。【方法】以相同饲养管理条件下同龄已有产羔记录的蒙古羊（单胎母羊和双胎母羊）、无角陶赛特羊（单胎母羊和双胎母羊）、小尾寒羊（多胎母羊）为试验材料，采用PCR-SSCP结合DNA直接测序的方法，对BMPR-IB基因第7外显子进行多态性检测与分析。【结果】试验羊只存在AA型和AB型2种基因型；AB型个体在BMPR-IB基因第864位点发生C→T突变，出现T/C的杂合，所有试验样本的优势基因型均为AA型，优势等位基因均为A基因。χ2适合性检验表明，无角陶赛特羊、蒙古羊和小尾寒羊均处于Hardy weinberg平衡状态，BMPR-IB基因第7外显子不同基因型在不同产羔类型绵羊中的分布差异显著，最小二乘法分析表明，绵羊BMPR-IB第7外显子多态性与产羔数有一定的相关性。【结论】BMPR-IB基因第7外显子第864位点的突变在不同繁殖力母羊群体中分布不同，表明绵羊BMPR-IB基因第7外显子多态性与绵羊产羔数相关，可以作为控制绵羊繁殖力的潜在遗传标记位点进行下一步研究。 |
| 关键词: 绵羊 BMPR-IB基因第7外显子 PCR-SSCP DNA直接测序 多态性 产羔数 |
| DOI： |
| 分类号: |
| 基金项目:甘肃省农牧厅生物技术专项（GNSW-2012～24）；国家自然科学基金（地方基金）项目（31260547） |
|
| Correlation between polymorphism of BMPR-IB gene exon7 and lambing performance of sheep |
|
JIA Jian-lei,ZHANG Li-ping,DING Qiang,et al
|
| Abstract: |
| 【Objective】This study aimed to explore the correlation between polymorphism of BMPR-IB gene exon7 and lambing performance in sheep and find locus to control reproduction of sheep.【Method】The breeding ewes including Mongolia ewes,Small Tail Han ewes and Poll Dorest ewes with different lambing performances (e.g.multiples,twins and singletons) were selected for studying polymorphism of BMPR-IB gene exon7 detected by PCR-SSCP and DNA sequencing.【Result】AA and AB genotypes were found in tested ewes.AB genotype had C→T mutation in coding region of 864 with heterozygous T/C.Genotype AA was dominant genotype and allele A was dominant allele in all samples.All sheep were in Hardy weinberg equilibrium based on χ2 test.There were significant differences in distribution of different genotypes of BMPR-IB gene exon7 in different sheep types.Least squares analysis showed that there was correlation between polymorphism of BMPR-IB gene exon7 and lambing performance.【Conclusion】There was correlation between polymorphism of BMPR-IB gene exon7 and lambing performance of sheep.The mutation in locus 846 of BMPR-IB gene exon7 could be considered as a potential locus to control reproduction of sheep. |
| Key words: sheep BMPR-IB gene exon7 PCR-SSCP DNA sequencing polymorphism lambing performance |
