| 摘要: |
| 【目的】 研究秦川牛SIRT3基因的多态性及其对秦川牛体尺、肉用性状的影响，以探索改善秦川牛体尺、肉用性状的分子育种方法。【方法】随机选择相近饲喂条件下468头18～24月龄健康秦川母牛，采用DNA直接测序技术进行SIRT3基因全区域遗传变异检测。运用SPSS16.0软件中最小二乘法拟合线性模型，对SIRT3基因不同基因型与秦川牛体尺(体斜长、体高、腰高、尻长、腰角宽、胸深、胸围和坐骨端宽)和肉质性状(背膘厚、眼肌面积和肌肉脂肪含量)进行关联分析。【结果】经DNA测序发现，秦川牛SIRT3基因在第4内含子上存在2个突变位点，分别为C22522T突变和C22680G突变，均存在3种基因型。χ2检验表明，C22522T和C22680G位点均处于Hardy Weinberg极度不平衡状态(P＜0.01)。关联性分析表明，秦川牛SIRT3基因2个突变位点的不同基因型与体斜长、体高、尻长、腰角宽、胸深、胸围和背膘厚、眼肌面积、肌肉脂肪含量有显著或极显著相关关系。【结论】SIRT3基因对秦川牛部分体尺、肉质性状有显著的影响，可以尝试作为秦川肉牛新品系培育的主要候选基因。 |
| 关键词: 秦川牛 SIRT3基因 SNPs 体尺性状 肉质性状 |
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| 基金项目:“十二五”国家“863”计划项目（2013AA102505，2011AA100307-02）；国家科技支撑计划项目（2011BAD2804-03）；国家自然科学基金项目（31272411）；国家转基因育种专项（2011ZX08007-002）；国家肉牛牦牛产业技术体系项目（CARS-38）；陕西省科技统筹创新工程计划项目（2011KTCL02-07） |
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| Association of SIRT3 gene polymorphism with body measurements and meat traits of Qinchuan cattle |
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DENG Guan-qun,ZAN Lin-sen,WANG Hong-bao,et al
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| Abstract: |
| 【Objective】The study investigated the polymorphism of SIRT3 gene and the association with body measurements and meat traits of Qinchuan cattle to find molecular breeding methods for improving body measurements and meat traits of Qinchuan cattle.【Method】In this study,468 Qinchuan heifers with the age of 18 to 24 months under similar feeding conditions were randomly selected.Direct DNA sequencing methods were used to detect the polymorphisms in SIRT3 gene.Least squares fitting linear model incorporated in SPSS16.0 program was used to analyze the relationship of genotype with body measurements and meat traits.【Result】Two SNPs,C22522T and C22680G,were detected in SIRT3 gene.PCR-RFLP analysis showed that three genotypes were found in C22522T and C22680G,respectively.χ2 test showed C22522T and C22680G were in Hardy Weinberg disequilibrium (P＜0.01).The genotypes of different mutations in SIRT3 gene were found to be significantly related to body length,withers height,hip height,rump length,pin bone width and back-fat thickness,lion muscle area,and intramuscular fatty content.【Conclusion】SNPs of the SIRT3 gene had significant effects on body measurements and meat traits of Qinchuan cattle.Thus,it could be used as a major candidate gene for new strain breeding of Qinchuan cattle. |
| Key words: Qinchuan cattle SIRT3 gene polymorphism body measurement meat trait |
