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猕猴桃抗溃疡病基因连锁SSR分子标记初步研究
易盼盼1, 樊红科2, 雷玉山,等2
1.西北农林科技大学 园艺学院;2.陕西省农村科技开发中心
摘要:
【目的】研究与猕猴桃抗溃疡病基因连锁的SSR分子标记,为猕猴桃抗病育种提供早期分子筛选标记。【方法】以易感病的雌株西选二号和抗病的雄株SX45872杂交F1代的198株群体以及40份猕猴桃材料为试材,利用离体接种法进行抗性鉴定。利用SSR(Simple sequence repeat)技术,结合集群分类分析法 (Bulked segregation analysis,BSA)进行猕猴桃抗溃疡病基因(PR)分子标记筛选。【结果】抗性鉴定结果表明,猕猴桃溃疡病抗/感病分离比符合由1对主效基因控制的1∶1分离比例。通过对51对SSR引物的筛选,获得了与抗溃疡病基因连锁的SSR分子标记UDK97-428116;将获得的SSR标记在40份猕猴桃材料中进行验证,分子标记结果与离体枝条接种结果一致率达95.5%,从而验证了该标记的可靠性。【结论】SSR标记UDK97-428116可用于猕猴桃材料溃疡病抗性鉴定和分子标记辅助育种。
关键词:  猕猴桃  溃疡病  抗性基因  SSR标记  离体接种
DOI:
分类号:
基金项目:陕西省科技统筹创新工程项目(2011KTCL02-06);陕西省农业科技创新项目(2012NKC01-05);陕西省猕猴桃产业提升关键技术研究与产业基地建设项目(2012GB2G000438)
Priliminary study on SSR marker of gene linkage against Pseudomonas syringae pv.actinidae
YI Pan-pan,FAN Hong-ke,LEI Yu-shan,et al
Abstract:
【Objective】The study investigated SSR marker of gene linkage against kiwifuit canker disease to provide molecular marker for breeding of kiwifruit with resistance.【Method】198 progenies in F1 generation of the susceptible female Xixuan No.2 and the disease resistance male plant SX45872 and 40 kiwifruit materials were selected for identification of resistance using in vitro inoculation method.Then simple sequence repeat (SSR) technique combined with bulk segregation analysis (BSA) was used to conduct marker screening of canker disease resistance gene (PR) in kiwifruit.【Result】The identification of resistance showed that the separation of kiwifruit canker disease resistance was in accordance with the 1∶1 segregation ratio controlled by a pair of main effect genes.A total of 51 primer pairs were screened,and an SSR marker (UDK97-428116) closely linked to PR gene was identified.The validity of this SSR marker was verified in 40 kiwifruit materials with the consistent rate of 95.5% compared to SSR in vitro inoculation branches.【Conclusion】The SSR marker UDK97-428116 can be used as molecular marker to assist selection of resistance to kiwifruit canker.
Key words:  kiwifruit  canker  resistance gene  SSR marker  in vitro inoculation