| 摘要: |
| 【目的】研究抗呼吸型IBV HI抗体水平与蛋鸡产蛋率的对应关系。【方法】将180只SPF鸡分为4组，第1组和第2组首免用鸡传染性支气管炎H120活疫苗免疫，1羽份/只，4周后采血，第2组鸡同时用3批次的鸡新城疫、传染性支气管炎、禽流感（H9亚型）三联灭活疫苗（La Sota株+M41株+HN6株）分别进行加强免疫，试验同时设置攻毒对照第3组和不免疫攻毒对照第4组。灭活疫苗免疫4周后，当产蛋率稳定在80%以上时，对试验鸡采血测定抗体，同时使用M41株病毒进行攻毒，记录攻毒后4周内各组鸡的产蛋性能，分析抗IBV HI抗体水平与攻毒后产蛋性能的相关性。【结果】弱毒活疫苗和灭活疫苗联合免疫组抗IBV HI抗体水平在2-8.2～2-9.3时，免疫鸡能够耐受IBV强毒的攻击，其产蛋性能不受影响。弱毒活疫苗免疫组抗体为2-4.8，产蛋量下降了12.69%。攻毒对照组抗体水平为2-2.0，攻毒后产蛋量下降45.22%。【结论】蛋鸡抗IBV HI抗体效价与产蛋率对鸡传染性支气管炎强毒的攻毒保护之间呈正相关，可以用HI抗体检测来评价鸡传染性支气管炎灭活疫苗对产蛋鸡的免疫效果。 |
| 关键词: IBV HI抗体效价 攻毒 产蛋性能 |
| DOI： |
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| 基金项目:河南省科技创新人才计划项目（144100510009）；郑州市前沿技术研究开发项目(141PQYJS566) |
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| Relationship between HI antibody titers against infectious bronchitis virus and egg production rate |
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LI Xin-sheng,JIAO Xian-qin,GAO Wen-ming,et al
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| Abstract: |
| 【Objective】This paper studied the relationship between HI antibody titers against infectious bronchitis virus strain and egg production rate.【Method】180 SPF chickens were divided into 4 groups.Chickens in the first and second groups were immunized with H120 live vaccine.4 weeks later,the second group chickens were vaccinated with Newcastle disease,infectious bronchitis,and avian influenza virus (H9 subtype) triple inactivated vaccine (La Sota+M41+HN6 strain).Group 3 and Group 4 had no immunization.4 weeks after vaccine was inoculated,the antibody in blood of tested chickens was determined when the egg production rate was stably over 80%.Then,the first three groups were challenged by virulent infectious bronchitis virus M41 strain,and chicken laying performance and egg quality were recorded in the following 4 weeks.Then the relationship between HI antibody titers against infectious bronchitis virus strain and egg production performance was analyzed.【Result】Chicken egg laying performance was not affected by virulent infectious bronchitis virus when their HI antibody titers against virulent infectious virus were 2-8.2－2-9.3.The first group which was only immunized by live attenuated vaccine had the HI titers of 2-4.8,and the egg production rate decreased by 12.69%.The HI tiers of control group were 2-2.0,and egg production rate decreased by 45.22%.【Conclusion】HI antibody titers and egg production performance were positively related.HI antibody detection can be used to evaluate the immune efficacy of inactivated vaccines with infectious bronchitis virus. |
| Key words: IBV HI antibody titer challenge egg production |
