| 摘要: |
| 【目的】建立快速检测副猪嗜血杆菌抗体的平板凝集试验方法，以便能够对副猪嗜血杆菌病进行快速、准确的诊断。【方法】利用9株(Ea、Ec、Ew、A1、A2、B1、B2、C1、C2)副猪嗜血杆菌陕西分离株制备平板凝集抗原，用其免疫健康家兔制备血清抗体，建立副猪嗜血杆菌平板凝集检测方法，对制备抗原的优势菌株进行筛选，并对该方法的特异性、敏感性、抗原最佳工作浓度进行检测。【结果】选出Ew株作为制备抗原的种子菌株，成功建立了检测副猪嗜血杆菌抗体的平板凝集试验方法；该方法具有良好的特异性和敏感性，抗原最适工作浓度为8×109 CFU/mL。采用本试验建立的副猪嗜血杆菌平板凝集试验方法对临床血清样品的检测结果与临床诊断结果基本一致。【结论】建立的副猪嗜血杆菌平板凝集试验方法能够对副猪嗜血杆菌病做出较为快速、准确的诊断；陕西省存在副猪嗜血杆菌不同程度的感染，规模猪场集中饲养的猪感染副猪嗜血杆菌的比率明显高于散户。 |
| 关键词: 副猪嗜血杆菌 抗原制备 平板凝集 抗体检测 |
| DOI： |
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| 基金项目:陕西省农业科技创新项目(2011NXC01-10) |
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| Establishment and application of plate agglutination method for detecting antibody against Haemophilus parasuis |
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GAO Yan,ZHANG Ding-quan,YANG Zeng-qi,et al
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| Abstract: |
| 【Objective】This study aimed to establish a rapid method for detecting antibody of Hps and prepare the plate agglutination antigen for the detection of serum Hps antibody.【Method】9 Hps strains (Ea,Ec,Ew,A1,A2,B1,B2,C1,and C2) stored in the infectious disease lab were revived and serum antibodies of immunizing rabbits was prepared.Then the plate agglutination method for detecting serum Hps antibody was established and the specificity,sensitivity,and best working concentration were decided.【Result】Ew strain was selected as the strain for generating antigen.The plate agglutination method for detecting antibody against Haemophilus parasuis was successfully established.The method had good specificity and sensitivity,and the suitable working concentration of antigen was 8×109 CFU/mL.The test results of clinical serum samples with the clinical diagnosis were basically the same.【Conclusion】The established plate agglutination method of Hps measurement diagnosed Gl-sser’s disease more rapidly and accurately.The results also showed that the Hps infection rate of scale pig farm was higher than that of scattered farms in Shaanxi. |
| Key words: Haemophilus parasuis antigen preparation agglutination antibody detection |
