| 摘要: |
| 【目的】分析位置候选基因SLCO1C1与绿壳蛋形成的关系，为绿壳蛋鸡提纯提供参考。【方法】以江西东乡绿壳蛋鸡为研究对象，以同群体的褐壳蛋鸡为对照，检测SLCO1C1基因上游6.5 kb区域内的序列变异情况；采用Real-time PCR法检测SLCO1C1在蛋鸡脑、肝、脾、蛋壳腺中的表达情况，用3′ RACE法克隆SLCO1C1 3′UTR，用PCR-RFLP法检测Ala528Glu错义突变基因型。【结果】在SLCO1C1基因上游6.5 kb区域内共发现了33个单核苷酸变异（SNP），这些SNP与绿壳性状显著关联(P＜0.05)。表达结果显示，SLCO1C1基因只在蛋鸡脑中表达，且在绿壳蛋鸡(n=5)与褐壳蛋鸡(n=5)间的表达无显著差异(P＞0.05)。序列比对结果显示，绿壳蛋鸡与褐壳蛋鸡的SLCO1C1 3′ UTR同源性为100%。在SLCO1C1基因11号外显子上发现了一个错义突变Ala528Glu， Glu突变与绿壳性状显著关联(P＜0.05)，且Glu突变在其他产绿壳蛋的鸟类中也存在。【结论】SLCO1C1可能通过间接途径影响胆绿素的转运，从而影响绿壳蛋的形成。SLCO1C1基因内存在大量的与绿壳性状显著关联的SNP，表明控制绿壳性状的O基因很可能就在SLCO1C1附近。 |
| 关键词: 鸡 绿壳蛋 位置候选基因 SLCO1C1 |
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| 基金项目:西北农林科技大学博士科研启动基金项目(Z109021112)；西北农林科技大学动物育种专项(Z109021127) |
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| Relationship of SLCO1C1 gene and formation of chicken blue-shelled egg |
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| Abstract: |
| 【Objective】Relationship of a position candidate gene SLCO1C1 and blue egg gene (O) was analyzed in this study to improve breeding of blue-shelled chicken.【Method】Dongxiang blue-shelled chicken were selected as study material and brown-shelled chicken from the same group were used as control.Sequence variants in upstream 6.5 kb of SLCO1C1 were detected by DNA sequencing.Expression characters of SLCO1C1 in brain,liver,spleen and shell gland were analyzed by Real-time PCR.3′ UTR was cloned by 3′ RACE.Genotype of a missense mutation in exon 11 of SLCO1C1 was detected by PCR-RFLP.【Result】A total of 33 SNPs were found in upstream 6.5 kb of SLCO1C1.All SNPs showed strong association with blue-shelled egg (P<0.05).Real-time PCR showed that the gene was uniquely expressed in brain and there was no significant difference between blue-shelled chickens (n=5) and brown-shelled chickens (n=5).3′ UTR of SLCO1C1 was firstly cloned and showed 100% sequence identity.A missense mutation Ala528Glu in exon 11 also showed strong association (P<0.05) with blue shelled egg and high conservation among birds laying blue-shelled egg.【Conclusion】SLCO1C1 may indirectly influence formation of blue-shelled egg.A large amount of SNPs associated with blue-shelled egg in SLCO1C1 implied that the O gene was most likely in vicinity of SLCO1C1. |
| Key words: chicken blue egg position candidate gene SLCO1C1 |
