| 摘要: |
| 【目的】探寻影响兔肉中脂肪酸性状的优势基因型。【方法】利用PCR-RFLP技术检测60只美系獭兔、51只伊拉肉兔FABP4基因的单核苷酸多态性，分析其与兔肉中脂肪酸含量的相关性。【结果】2个兔品种在intron1中存在C/T突变，该突变位点能被TaiⅠ限制性酶识别，且经酶切后发现，2个兔群体中存在CC、CT和TT 3种基因型。美系獭兔的3种基因型频率分别为0.083，0.467和0.450，等位基因C和T的基因频率分别为0.683和0.317；伊拉肉兔的3种基因型频率分别为0.137,0.549和0.314，等位基因C和T的基因频率分别为0.588和0.412。关联分析结果表明，在美系獭兔群体中，CC型个体肌肉组织中的脂肪酸C14:0含量和脂肪酸C18：1，n-9含量显著高于CT和TT型个体；TT型个体脂肪酸C18:3，n-3含量显著高于CC和CT型个体；TT型个体脂肪酸C20:4，n-6含量显著高于CC型个体。在伊拉肉兔群体中，CC基因型个体肌肉组织中的C18:1，n-9含量显著高于CT和TT基因型个体(P＜0.05)；CC型个体脂肪酸C18:2，n-6含量显著高于CT型个体；TT型个体脂肪酸C18:3，n-3含量显著高于CC和CT型个体；CT型个体脂肪酸C20:4，n-6含量显著高于CC型个体。【结论】FABP4基因可作为美系獭兔和伊拉肉兔脂肪酸性状选育的候选基因。 |
| 关键词: 美系獭兔 伊拉肉兔 脂肪酸 FABP4基因 RFLP |
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| 基金项目:陕西省农业攻关项目(2010K01-16)；西安市农业攻关项目(NC09049-4) |
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| Correlation analysis of FABP4 gene polymorphism and meat fatty acid content between Rex and IRA rabbit |
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| Abstract: |
| 【Objective】This study aimed to find the dominant genotype influencing fatty acid character in rabbit meat.【Method】FABP4 gene single nucleotide polymorphism of 60 Rex rabbits and 51 IRA rabbits was detected with PCR-RFLP,and the correlation between SNP and fatty acid content of the two rabbits was analyzed.【Result】The intron1 C/T mutation existed in both rabbit breeds,which can be recognized by TaiⅠ restriction enzymes.There were three genotypes:CC,CT,and TT.The genotype frequencies of Rex were 0.083,0.467 and 0.450,and the allele gene frequencies of C and T were 0.683 and 0.317.The genotype frequencies of IRA were 0.137,0.549 and 0.314,and the allele gene frequencies of C and T were 0.588 and 0.412.Correlation analysis showed that for Rex rabbits,the content of fatty acid C14:0 and C18:1,n-9 in CC genotype was significantly higher than that of CT and TT genotypes (P<0.05);C18:3,n-3 contents of CC and CT were significantly lower than that of TT genotype;and C20:4,n-6 content of TT genotype was significantly higher than that of CC.For IRA rabbits group,the fatty acid C18:1,n-9 content of CC genotype was significant higher than that of CC and CT genotypes (P＜0.05);C18:2,n-6 content of CC genotype was significantly higher than that of CT genotype;C18:3,n-3 content of TT genotype was significantly higher than that of CT and CC genotypes;and C20:4,n-6 content of CT genotype was significantly higher than that of CC genotype.【Conclusion】FABP4 gene can be used as candidate gene for fatty acids character of Rex and IRA rabbits. |
| Key words: Rex rabbits IRA rabbits fatty acids FABP4 gene RFLP |
