| 摘要: |
| 【目的】对中国河西绒山羊、内蒙古绒山羊、辽宁绒山羊和陇东绒山羊4个绒山羊群体KAP13-1基因的遗传特征进行分析，为揭示绒山羊遗传特征和生产利用提供基础数据。【方法】采用PCR-SSCP方法，检测河西绒山羊、内蒙古绒山羊、辽宁绒山羊及陇东绒山羊（共721只）KAP13-1基因的多态性。同时基于DA遗传距离用UPGMA法进行聚类分析，以明确各群体间的遗传关系。【结果】基因组DNA扩增后得到491 bp的扩增产物，经SSCP分析，从4个绒山羊群体KAP13-1基因中共发现6种基因型，4个等位基因中存在5个变异位点，这些多态位点主要为点突变，且均处于编码区，其中转换位点3个(占60%)，颠换位点2个(占40%)。在170和197位分别发生了T→G、C→G的错义突变，导致氨基酸分别由Leu、Thr变为Arg、Ser。该基因在河西绒山羊与陇东绒山羊中均存在4个等位基因，而内蒙古绒山羊未检测到C和D等位基因，辽宁绒山羊未检测到D等位基因。聚类结果显示，河西绒山羊与陇东绒山羊之间的遗传距离较小，在系统发生树中聚为一支，辽宁绒山羊与内蒙古绒山羊聚为一支。【结论】KAP13-1基因在4个绒山羊群体中呈中度多态，群体间存在差异。生态学作用对4个绒山羊品种的进化过程有一定的影响。 |
| 关键词: KAP13-1基因 绒山羊 PCR-SSCP 遗传多态性 |
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| 基金项目:甘肃省科技重大专项(1102NKDM024)；2012年甘肃省高等学校基本科研业务费项目；甘肃省青年科技基金计划项目（1107RJYA060） |
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| Genetic polymorphism of KAP13-1 gene of four cashmere goat populations in China |
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| Abstract: |
| 【Objective】The objectives of this study were to analyze the genetic variations of KAP13-1 gene of Hexi cashmere goat,Inner Mongolia cashmere goat,Liaoning cashmere goat and Longdong cashmere goat,and provide data for future studies on cashmere traits.【Method】KAP13-1 genes were amplified using PCR-single stranded conformational polymorphism (PCR-SSCP) analysis to detect genetic variations in 721 cashmere goats (Hexi cashmere goat,Inner Mongolia cashmere goat,Liaoning cashmere goat,Longdong cashmere goat) in China.Then we constructed UPGMA tree by genetic distances (DA).【Result】The PCR amplification obtained KAP13-1 gene with 491 bp,and six genotypes were revealed from the four cashmere goat populations.Five nucleotide polymorphism sites,which mainly came from point mutation,were identified in four alleles.There were three transition sites (60%) and two transversion sites (40%).The mutation of 170 and 197 T→G,C→G resulted in Leu→Arg and Thr→Ser missense mutation.Four alleles were found in Hexi and Longdong cashmere goat populations,while there was no alleles C and D in Inner Mongolia cashmere goats,and allele D had not been detected in Liaoning cashmere goat populations.Clustering results showed that Hexi and Longdong cashmere goat populations clustered together firstly,and then Liaoning and Inner Mongolia cashmere goat populations clustered together.【Conclusion】All of the four cashmere goat populations are moderately polymorphic in KAP13-1 gene,and there are rich genetic diversities among the four cashmere goat populations.Ecological factors have certain effects on the evolution of these four cashmere goat breeds. |
| Key words: KAP13-1 gene cashmere goat PCR-SSCP genetic polymorphism |
