| 摘要: |
| 【目的】分析奶山羊间性个体的遗传背景，探讨间性个体发生的遗传机制，为山羊性别调控以及性别决定的研究提供参考。【方法】通过核型分析、特异性性别调控基因(Sex-determining region of Y chromosome，SRY )的PCR扩增以及AFLP(Amplified fragment length polymorphism)指纹图谱分析等方法，研究西农萨能羊间性个体的遗传本质。【结果】间性山羊与正常母山羊的核型一致，为58+XX；间性个体基因组中未能扩增出SRY基因；AFLP指纹图谱分析发现，有9对引物扩增出清晰的条带，共1 833条，片段长度为70～500 bp，9对引物的扩增结果差异明显，表现出丰富的多态性，共获得611个多态位点，占总扩增条带数的33.3%，但在基因组水平上间性山羊与正常母山羊个体并没有大的差异。【结论】根据试验结果推测，间性山羊的出现并非是由个体基因组发生突变造成的，可能是在性别决定过程中某个因子的调控出现差错，从而导致胚胎在发育过程中偏离雌性轨道而产生间性山羊个体，其具体机制有待进一步研究。 |
| 关键词: 间性山羊 核型分析 SRY基因 AFLP指纹图谱 |
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| 基金项目:公益性行业(农业)科研专项(201103038)；陕西省重大科技创新项目(2009ZKC07-01) |
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| Genetic determination and AFLP fingerprinting analysis of intersex dairy goats |
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| Abstract: |
| 【Objective】The research was conducted to investigate the development mechanism of intersex goat by analyzing its genetic background.【Method】The paper studied the genetic characteristics of intersex goats through karyotype analysis,amplification of SRY (Sex-determining region of Y chromosome) gene and AFLP (Amplified fragment length polymorphism) fingerprinting.【Result】The results showed that the karyotype of intersex goats was 58+XX,same as that of normal goats.SRY gene was not found in genome of intersex goats.AFLP fingerprinting found that there were nine pairs of primers amplified clear bands and the total number was 1 833 with the size of 70-500 bp.The differences between the nine pairs of primers were distinct,showing various polymorphisms.A total of 611 polymorphic loci were obtained,accounting for 33.3% of the whole amplified bands.However,there was no large difference between intersex and normal dairy goats in genome.【Conclusion】According to the results,it was predicted that the occurrence of intersexuality was not resulted from mutations in individual genome.Instead,it may be caused by failing regulation of some factors in the process of sex determination,which leads the deviation of individual embryo from the female track during the development.Further work should be done to clarify the mechanism in. |
| Key words: intersex goats analysis of karyotype SRY gene AFLP fingerprinting |
