| 摘要: |
| 【目的】对甘南牦牛NGB基因进行克隆和序列分析，同时探讨NGB的生理功能。【方法】采用TA克隆法克隆甘南牦牛NGB基因，运用多种软件对其基因序列及编码产物的结构、功能进行了生物信息学分析。【结果】甘南牦牛NGB基因全长3 844 bp，包括4个外显子和3个内含子，其内含子中存在2个反向重复序列和1个正向重复序列；牦牛NGBCDs区全长456 bp，其编码产物是由151个氨基酸残基组成的可溶性蛋白质，分子质量约为16 876.36 u，理论等电点为4.86，推测GB编码产物可能在牦牛物质运输和结合、能量代谢等过程中发挥着重要作用；系统发育树分析表明，甘南牦牛NGB与牛、藏羚羊等物种之间存在较高的同源性。【结论】甘南牦牛NGB基因的成功克隆，为进一步研究牦牛NGB的遗传特性和生理功能奠定了基础。 |
| 关键词: 甘南牦牛 脑红蛋白 基因克隆 生物信息学分析 |
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| 基金项目:甘肃省自然科学基金项目(1107RJZA148)；甘肃省教育厅研究生导师项目(1102-04) |
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| Cloning and sequence analysis of NGB gene of Gannan yak |
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| Abstract: |
| 【Objective】In this study,the NGB gene of Gannan yak was cloned and its sequence was analyzed.The physiological function of the NGB was explored as well.【Method】NGB gene of Gannan yak was cloned by TA cloning.The NGB gene sequence,structure,function and its encoded amino acid sequence were analyzed with biological information.【Result】The full-length of NGB gene was 3 844 bp,including four exons and three introns.There were two inverted repeat sequences and a direct repeat sequence in intron.The coding region of NGB gene was 456 bp,encoding a protein of 151 amino acids with a molecular weight of 16 876.36 u and pI of 4.86.The deduced encoded product was a soluble protein,playing an important role in the process of transport,binding and energy metabolism.Phylogenetic analysis showed that there was a higher homology between Gannan yak and Bos taurus,Pantholops hodgsonii.【Conclusion】This study paved the way for future study of genetic characteristics and physiological mechanism of NGB gene of Gannan yak． |
| Key words: Gannan yak neuroglobin gene cloning biological information analysis |
