| 摘要: |
| 【目的】探讨毛果杨(Populus trichocarpa)全基因组中铵转运蛋白(AMTs)家族成员的系统发育及部分成员的理化性质、结构和亚细胞定位。【方法】以从毛果杨全基因组数据库中搜索并筛选得到的目标蛋白序列为基础，应用软件CLUSTALX 2.0、GeneDOC和MEGA4,对AMTs家族成员的系统发育进行了分析，并重点分析了AMT1亚家族成员PtrAMT1-1、PtrAMT1-6和AMT2亚家族成员PtrAMT2-1、PtrAMT4-5的保守基序、理化参数、亲/疏水性、跨膜域、三级结构及亚细胞定位。【结果】在毛果杨基因组数据库中发现了19个AMTs，8个属于AMT1亚家族，11个属于AMT2亚家族；AMT1和AMT2亚家族内的蛋白序列保守性强，而亚家族之间的差异较大；AMT1与AMT2间的共同基序较少；AMTs是一类位于膜上运输NH4+的蛋白家族，有10～11个跨膜域，亚家族内蛋白三维结构相似，不同AMTs成员的亚细胞定位不同。【结论】在毛果杨中，AMT1与AMT2亚家族分开较早，各AMTs在杨树氮素代谢中起着不同的作用，共同维持并调控着杨树体内的氮素平衡。 |
| 关键词: 铵转运蛋白 杨树 基因组 序列分析 |
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| 基金项目:教育部新世纪优秀人才支持计划项目（NCET-08-0468）；教育部高等学校博士学科点专项科研基金项目（20090204110027）；霍英东教育基金会项目（121026） |
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| Genome wide analysis of the ammonium transporter gene family in Populus trichocarpa |
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| Abstract: |
| 【Objective】This study aims to elucidate the phylogenesis of the ammonium transporter (AMTs) family members and to analyze the physical,chemical,structural characteristics and sub cellular location of four ammonium transporters (PtrAMT1-1,PtrAMT1-6,PtrAMT2-1 and PtrAMT4-5) from Populus trichocarpa.【Method】Phylogenetic analysis of AMTs from the sequenced genome of P.trichocarpa was performed by using softwares CLUSTALX 2.0,GeneDOC and MEGA4.The physical,chemical,hydrophilicity/hydrophobicity,transmembrane domains,secondary and three dimensional structure were analyzed by online softwares of bioinfomatics.【Result】Nineteen AMTs are found in the genome of P.trichocarpa.Eight PtrAMTs belong to AMT1 subfamily and the other eleven to AMT2 subfamily.Within PtrAMT subfamily the amino acid sequence is highly conserved,but marked difference in amino acid sequence exists between PtrAMT1 and PtrAMT2.There are few common motifs between PtrAMT1 and PtrAMT2.PtrAMTs are proteins transporting ammonium ions across cell membranes.PtrAMTs contain 10-11 transmembrane domains. Within PtrAMT subfamily the three dimensional structures of PtrAMTs are similar.PtrAMT family members distribute different sub cellular locations.【Conclusion】These results indicate that PtrAMT1 and PtrAMT2 may be separated in an early stage during their evolution processes and PtrAMT family members play different roles in nitrogen metabolism to maintain the nitrogen homeostasis in P.trichocarpa under changing nitrogen conditions. |
| Key words: ammonium transporters Populus trichocarpa genome sequence analysis |
