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桃叶片再生不定芽的研究
田国栋, 张荷芃, 康卓慧
西北农林科技大学 园艺学院
摘要:
【目的】研究桃叶片再生不定芽的影响因子,建立并优化叶片再生体系。【方法】以桃品种“布目早生”、“甜桃王”、“瑞江”和优系“97-8-4”组培苗叶片为试材,研究基因型、叶龄、叶片不同部位、基本培养基及生长调节剂对不定芽再生的影响。【结果】不同基因型叶片的不定芽再生率差异较大,“97-8-4”和“甜桃王”不定芽再生率均较高,“布目早生”次之,“瑞江”的叶片不能再生;叶片在不同培养基中再生不定芽的效果不同,以LP培养基的再生效果较好;不同成熟度的叶片再生不定芽的能力不同,完全展开幼嫩叶片的再生效果较好;叶片不同部位产生不定芽的能力不同,叶柄和叶片基部的再生效果较好;叶片暗培养时最适的生长调节剂组合为2.0 mg/L BA+0.2 mg/L NAA,光培养时最适的BA质量浓度为4.0~6.0 mg/L,BA与NAA适宜的质量浓度比为30∶1~60∶1。“97-8-4”、“甜桃王”和“布目早生”完全展开的幼嫩叶片在最适培养条件下的不定芽再生率分别为18.33%,16.67%和13.33%。【结论】 桃叶片再生不定芽受内外因素的共同影响,基因型和叶片外植体的生理状态是不定芽再生的关键内在因素,培养基及附加的生长调节剂是不定芽再生的关键外在因素。
关键词:    叶片  离体再生  不定芽
DOI:
分类号:
基金项目:陕西省“13115”科技创新工程重大科技专项(2008ZDKG-08);陕西省科技攻关项目(2008K01-11);西北农林科技大学唐仲英育种基金项目
Study on shoot regeneration from leaf explants in peach
Abstract:
【Objective】Factors affecting shoot regeneration from leaf explants in vitro were investigated in peach in order to establish and optimize shoot regeneration system.【Method】Experiment was conducted with “Bumuzaosheng”,“Tiantaowang”,“Ruijiang” and “97-8-4” as test materials to study the effects of different genotypes,and different leaf ages,different basic media,different growth regulators on shoot regeneration of leaf explants from tissue culture plantlets in peach.【Result】Shoot regenerating rate differed greatly from leaf explants in different genotypes.Leaf explants of “97-8-4” and “Tiantaowang” showed high shoot regeneration rate.Leaf explants of “Bumuzaosheng” were less efficient.Leaf explants of “Ruijiang” was unable to regenerate.Shoot regnerating rate was different in different basic media and LP was the optimum basic medium for shoot regeneration.Shoot regenerating rate differed from leaf explants in different maturity and the fully expanding young leaf was the optimum explant for shoot regeneration.Shoot regenerating rate was different from different parts of leaf explants.The petiole and the base of leaf were efficient for shoot regeneration.In dark culture the optimum growth regulators were 2.0 mg/L BA with 0.2 mg/L NAA for shoot regeneration.In dark light culture,the optimum growth regulators were 4.0-6.0 mg/L BA and the concentration of BA/NAA was 30∶1-60∶1.Shoot regenerating rate from fully expanding young leaf explants of “97-8-4”,“Tiantaowang” and “Bumuzaosheng” was 18.33%,16.67% and 13.33% under optimum culture condition,respectively.【Conclusion】Shoot regeneration was afftected by internal and external factors from leaf explants in peach.The genotype and the physiological state of leaf explants were the crucial internal factors for shoot regeneration.The basic medium and the growth regulators were the crucial external factors for shoot regeneration.
Key words:  peach  leaf explant  regeneration in vitro  shoot