| 摘要: |
| 【目的】研究菊科蒿属植物适应土壤干旱条件的抗氧化特性，为揭示生态恢复过程中植被演替的规律提供理论依据。【方法】以黄土高原演替初期阶段的猪毛蒿（Artemisia scoparia Waldst.et Kit.）、茭蒿（Artemisia giraldii Pamp.）、铁杆蒿（Artemisia gmelinii Web.ex Stechm.）3种优势种和1种常见种黄花蒿（Artemisia annua Linn.）为供试材料，采用盆栽试验，利用称质量控水法，将土壤含水量分别控制在田间最大持水量的75%（适宜水分对照）、55%（中度水分胁迫）和35%（重度水分胁迫），测定不同水分胁迫条件下4种菊科蒿属植物超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的活性,以及丙二醛（MDA）、抗坏血酸（Vc）、类胡萝卜素（Car）含量的变化，并采用隶属（反隶属）函数法对4种菊科蒿属植物的抗氧化特性进行了综合评价。【结果】在水分胁迫条件下，铁杆蒿具有相对最高的SOD活性和最低的POD活性；猪毛蒿的CAT、POD活性相对偏低，其余各项指标的活性和含量相对均衡，膜脂过氧化程度较低；茭蒿有相对最高的CAT活性、Vc含量、膜脂过氧化程度和最低的Car含量；黄花蒿有相对最高的POD活性、Car含量和相对最低的Vc含量、SOD活性、膜脂过氧化程度。4种蒿属植物铁杆蒿、猪毛蒿、茭蒿、黄花蒿在水分胁迫下的抗氧化特性隶属值分别为0.369，0.418，0.428，0.514。【结论】在水分胁迫条件下，4种菊科蒿属植物的抗氧化特性差异较大，综合评价认为，黄花蒿整体抗氧化能力最强，茭蒿、猪毛蒿次之，铁杆蒿最弱。 |
| 关键词: 菊科蒿属植物 抗氧化酶 抗氧化剂 抗旱性 |
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| 基金项目:中国科学院知识创新项目(KZCX2-XB2-05-01)；中国科学院重要方向项目（KZCX2-YW-443） |
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| Anti-oxidative responses to soil water stress for four Artemisia species |
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| Abstract: |
| 【Objective】Anti-oxidative responses of Artemisia species under drought environment were studied to reveal the drought resistence mechanism of vegetation in the succession process of ecological restoration.【Method】Artemisia scoparia Waldst.et Kit.,Artemisia giraldiiPamp.,and Artemisia gmelinii Web.ex Stechm.as three dominant species during early succession in the Loess Plateau,while Artemisia annua Linn.as conventional species,were selected to investigate physiological adaptation to soil water stress in the aspect of anti oxidative responses.Pot experiment was adopted to control the soil water content to the level as 75% of field moisture capacity (controlling water),55%(medial water stress),and 35%(severe water stress)respectively.During the period of water stress,the activity of SOD(superoxide dismutase),CAT(catalase),POD(peroxidase),MDA(malondialdehyde) content,Vc(vitamin C) content,and Car(carotenoid) content were measured periodically.And membership function was used to evaluate the anti oxidative capacity of four Artemisia species.【Result】Under water stress condition,the antioxidative indexes of 4 Atermisia species were analysed.The result showed that,A.gmelinii had maximum in SOD activity and minimum in POD activity;A.scoparia had relatively low activity in CAT,POD and MDA content;A.giradii had maximum in CAT activity,Vc content,MDA content and minimum in Car content;A.annua had maximum in POD activity,Car content and minimum in SOD activity,Vc content,MDA content.The membership function values of anti oxidative capacity of four Artemisia species were 0.369 (A.gmelinii),0.418(A.scoparia),0.428(A.giraldii) and 0.514(A.annua).【Conclusion】 Comprehensive analysis of the subordinate function showed that the anti oxidative capacity of four Artemisia species listed as A.annua＞A.giradii＞A.scoparia＞A.gmelinii. |
| Key words: Artemisia enzymatic antioxidant non enzymatic antioxidant drought resistance |
