| 摘要: |
| 【目的】 探讨僵蚕抑菌活性成分的提取方法，比较所得提取物对大肠杆菌的抑制作用，为解析和深入研究僵蚕的抑菌药理机制提供参考。【方法】 以僵蚕为材料,以体积分数95%乙醇为提取溶剂，对中药僵蚕的4种提取方法(超声波法、微波法、煎煮法、冷浸法)进行平行比较研究；采用超声波提取法，探讨了石油醚、二氯甲烷、乙酸乙酯、丙酮、体积分数95%乙醇、甲醇和水7种提取溶剂的提取效果，采用比色法、纸片扩散试验和肉汤倍比稀释法研究所得僵蚕活性提取物对大肠杆菌（Escherichia coli）的抑菌活性。【结果】 以体积分数95%乙醇作为提取溶剂，4种提取方法中，以超声波法提取时的浸膏得率最高。采用超声波提取法时，7种提取溶剂中，以体积分数95%乙醇提取浸膏的得率最高。研究结果显示，不同提取方法得到的提取物对大肠杆菌均有明显的抑菌作用，其中采用超声波提取法，以体积分数95%乙醇为提取溶剂时，所得提取物的抑菌活性最高，其对大肠杆菌的最低抑菌浓度（MIC）为0.625 mg/mL。【结论】 不同提取方法得到的僵蚕提取物对大肠杆菌均具有明显的抑菌活性，僵蚕的抗炎作用与其抑菌活性相关。 |
| 关键词: 僵蚕 活性成分提取 抑菌作用 大肠杆菌 |
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| 基金项目:国家自然科学基金项目（30500391,30872033） |
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| Analysis on antibacterial components from Bombyx batryticatus and its antibacterial activity against Escherichia coli |
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| Abstract: |
| 【Objective】 In this study,the methods of extracting the antibacterial component Bombyx batryticatus were tested,and the antibacterial effects of Bombyx batryticatus on Escherichia coli were determined in bioassay.【Method】 With Bombyx batryticatus as the material，the volume fraction of 95% ethanol as extraction solvent,parallel and comparative study was done on four kinds of extraction methods (ultrasonic,microwave method,boiling method,the cold soak method) of the traditional Chinese medicine Bombyx batryticatus.And the ultrasonic extraction method was taken to discuss extraction of seven kinds of extraction solvents,such as aether petroleum,dichloromethane,ethyl acetate,acetone,the volume fraction of 95% ethanol,methanol and water.Colorimetry,disk diffusion test and broth dilution were used to study the antibacterial activity of the obtained extracts of Escherichia coli.【Result】 With the volume fraction of 95% ethanol as extraction solvent among the four kinds of extraction methods,the ultrasonic extraction was the highest rate.Ultrasonic extraction method showed among the seven kinds of solvent extractions,the volume fraction of 95% ethanol extract had the highest rate of extraction.The results showed that different extractions had significant inhibitory effects on E.coli,and the extraction of using ultrasonic extraction method and the volume fraction of 95% ethanol showed the highest antimicrobial activity,the minimum inhibitory concentration (MIC) on Escherichia coliwas 0.625 mg/mL.【Conclusion】 Different extractions of Bombyx batryticatus all had significant antibacterial activity on E.coli,indicating that the anti inflammatory effect of Bombyx batryticatus was related to its antibacterial activity. |
| Key words: Bombyx batryticatus antibacterial component extract antibacterial activity Escherichia coli |
