| 摘要: |
| 【目的】 研究中药免疫调节剂对免疫抑制小鼠的组织保护作用。【方法】 用环磷酰胺（Cyclophosphamide,Cy）构建小鼠免疫抑制病理模型。分别给正常小鼠和环磷酰胺所致免疫抑制小鼠饮服自制中药免疫调节剂玉屏风散加减（ZY）和经典方剂玉屏风散（YPF），检测ZY、YPF对不同免疫状态下小鼠血液学指标（白细胞计数（WBC）、红细胞计数（RBC）、血红蛋白（HGB）、红细胞压积（HCT）、平均血红蛋白含量（MCH）、平均红细胞体积（MCV））、体质量、脾脏指数以及肝脾组织病理学的影响。【结果】 （1）ZY和YPF对健康小鼠血液学指标影响不显著（P>0.05）；ZY对Cy所致的免疫抑制小鼠WBC、RBC、HGB、HCT、MCH、MCV值有显著影响（P<0.05），YPF对Cy所致的免疫抑制小鼠RBC、HGB和MCH值有显著影响（P<0.05），且ZY的作用效果优于YPF（P<0.05）。（2）ZY和YPF对正常小鼠体质量及脾脏指数的影响不明显（P>0.05），而对Cy所致的免疫抑制小鼠体质量影响显著（P<0.05），小鼠的脾脏指数显著低于Cy所致的免疫抑制小鼠（P<0.05）。（3）ZY+Cy组和YPF+Cy组小鼠脾脏白髓大而明显，肝细胞轻度变性坏死；而模型对照组（Cy组）小鼠脾脏白髓不明显，肝细胞变性坏死严重。【结论】 ZY对正常小鼠血细胞数、体质量、脾脏指数的影响均不显著，而对免疫抑制小鼠血细胞数、体质量、脾脏指数以及脾、肝组织病理学均表现出一定的正向调节作用。 |
| 关键词: 中药免疫调节剂 环磷酰胺 免疫抑制 玉屏风散加减 |
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| 基金项目:安徽高校省级自然科学研究重点项目（KJ2009A033Z）；山东省中青年科学家科研奖励基金项目（2008BS07001） |
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| Protective effect of Chinese herbal medicine immunomodulator on tissues of immunosuppressed mice by cyclophosphamide |
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| Abstract: |
| 【Objective】 The study was to explore the protective effect of Chinese herbal medicine immunomodulator on the tissues of immunosuppressed mice. 【Method】 The immunosuppressed mice were induced by cyclophosphamide (Cy). The normal mice and immunosuppressive mice were administered separately with the Chinese herbal immunomodulator (ZY) and traditional prescription Yupingfeng (YPF),and then their hematological parameters（WBC，RBC，HGB，HCT，MCH and MCV）,body weight,spleen index and histopathological changes of liver and spleen in different immune status were detected. 【Result】 (1) Hematological parameters of healthy mice were not significantly affected by ZY and YPF (P>0.05);The hematological parameters(WBC,RBC,HGB,HCT,MCH and MCV) of immunesuppressive mice induced by Cy were significantly affected by ZY(P<0.05),the hematological parameters(RBC,HGB and MCH) of immunesuppressive mice induced by Cy were significantly affected by YPF (P<0.05),and ZY was better than YPF.(2) Body weight and spleen index of healthy mice were not significantly affected by ZY and YPF (P>0.05);But the immunosuppressed mice induced by Cy were significantly affected (P<0.05).(3) It showed that the splenic white pulp of mice in hispathologic slide was large and obvious and liver cells had mild degeneration and necrosis in group ZY+Cy and group YPF+Cy;splenic white pulp was not obvious and liver cells had a severe degeneration and necrosis in group Cy.【Conclusion】 ZY had remarkable effects on the hematological parameters,body weight and organ index of normal mice.However,it had significant positive effects on hematological parameters,body weight,organ index and pathology in the liver and spleen tissue of immunosuppressive mice. |
| Key words: Chinese herb immunomodulator cyclophosphamide immune suppression traditional prescription Yupingfeng |
