| 摘要: |
| 【目的】阐明生长素（Ghrelin）及其mRNA在大鼠卵巢和睾丸中的分布。【方法】 取成年SD大鼠的睾丸和卵巢，制成石蜡切片，采用免疫组化和原位杂交方法，分别检测Ghrelin和Ghrelin mRNA在大鼠性腺组织中的定位。【结果】 雄性大鼠睾丸间质细胞、支持细胞、初级精母细胞、次级精母细胞中均有Ghrelin分布， 而精原细胞、精子细胞、肌样细胞中均无Ghrelin分布；Ghrelin mRNA的分布规律与Ghrelin一致。在雌性大鼠卵巢中,Ghrelin主要分布在黄体细胞、卵巢门间质细胞、卵母细胞上；Ghrelin mRNA在黄体细胞、卵巢门间质细胞、原始卵泡、初级卵泡的颗粒层细胞、次级卵泡的卵泡膜层细胞和颗粒层细胞中均有分布。【结论】 Ghrelin及其mRNA在大鼠性腺中均有分布，可能对大鼠性腺的发育及其功能有一定的调控作用。 |
| 关键词: Ghrelin Ghrelin mRNA 免疫组化 原位杂交 大鼠 |
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| 基金项目:安徽省自然科学基金项目（070411015） |
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| Localization of Ghrelin and its mRNA in the rat gonads |
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| Abstract: |
| 【Objective】 The study was to investigate the distribution of ghrelin and mRNA in the ovary and testis of rat .【Method】 Adult SD rats testes and ovaries were taken to make paraffin sections.The localization and mRNA of ghrelin in gonadal tissues were detected by immunohistochemical PV-9000 2-step and in situ hybridization respectively.【Result】 The Ghrelin peptide was selectively expressed in Leydig cells,Sertoli cells,primary spermatocyte,and secondary spermatocytes.Ghrelin mRNA and ghrelin showed the same distribution in testis.In ovary,ghrelin was mainly distributed in the luteal cells,hilus interstitial cells and oocyte;while Ghrelin mRNA was expressed in luteal cells,hilus interstitial cells,the granulosa cells of primordial follicle and primary follicle,the granulosa and the thecal cells of secondary follicle.【Conclusion】 Ghrelin and Ghrelin mRNA were both present in rat gonads,which suggested that the ghrelin may play a role in development and function in rat gonads. |
| Key words: Ghrelin Ghrelin mRNA immunohistochemistry in situ hybridization rat |
