| 摘要: |
| 【目的】 研究大肠埃希氏细菌中超广谱β-内酰胺酶(Extended-spectrum β-lactamases,ESBLs)耐药质粒的传播及氯丙嗪对其的消除效果。【方法】 采集河南开封和安阳疑似大肠埃希氏细菌病病死鸡、猪典型病料，经细菌学检验后，用试管二倍稀释法测定头孢噻呋和头孢曲松对致病性大肠埃希氏细菌的最小抑菌浓度（MIC），用双纸片协同法和PCR扩增法进行ESBLs检测。以安阳猪和开封鸡大肠埃希氏细菌作供体菌、DH5α为受体菌，进行ESBLs耐药质粒的接合传递试验。用氯丙嗪作消除剂，以SDS为对照，对大肠埃希氏细菌分离株ESBLs耐药质粒进行消除试验。【结果】 从疑似大肠埃希氏细菌典型病料中分离到了大肠埃希氏细菌；头孢噻呋和头孢曲松对分离菌株的最小抑菌浓度分别为32～64和64～128 μg/mL；检测到了ESBLs耐药质粒的存在，且其能在大肠埃希氏细菌间传播，氯丙嗪对其第12次的消除率高达98.83%。【结论】 ESBLs耐药质粒可通过接合转移方式传递至感受态大肠埃希氏细菌中，氯丙嗪对其消除效果很好。 |
| 关键词: ESBLs 耐药质粒 大肠埃希氏细菌 |
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| 基金项目:河南省教育厅资助项目（2009A230009）；河南省科技厅资助项目（072102130009） |
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| Research on the spread and elimination of ESBLs in Escherichia coli |
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| Abstract: |
| 【Objective】 The spread of extended-spectrum β-lactamases resistant plasmids (ESBLs)in Escherichia coli and the elimination of Chlorpromazine were studied.【Method】 Suspicious materials of chicken and swine E.coli disease were collected from different chicken and swine flocks in 2 cities of Henan Province.After bacteria identification,the minimal inhibitory concentrations(MICs)of Cetiofur and Ceftriaxone to the Enterobacteriaceaes were carried out with two fold dilution method.ESBLs were detected by double-disk test and PCR method.Transformation and conjugation of E.coli resistant plasmids were done between donor and receiptor bacteria.Elimination of resistant plasmid experiment was tested by SDS and Chlorpromazine.【Result】 E.coli were isolated from Suspicious materials,the minimal inhibitory concentrations(MICs)of Cetiofur and Ceftriaxone to the E.coli were 32-64,64-128 μg/mL respectively;the ESBLs resistant plasmids were detected by double-disk test,ESBLs plasmid in E.coli can spread among bacteria,and the twelfth elimination rate of Chlorpromazine was 98.83%.【Conclusion】 The ESBLs resistant plasmids could be transferred to E.coli through conjugation and transformation.The elimination of Chlorpromazine to ESBLs resistant plasmids was good. |
| Key words: ESBLs resistant plasmid Escherichia coli |
