| 摘要: |
| 【目的】 研究一氧化氮（NO）对绿豆侧根发生的影响。【方法】 以绿豆为材料，用硝普钠（SNP）、NO特异清除剂c-PTIO和动物NOS抑制剂L NAME单独处理绿豆侧根或与IBA、NAA混合处理，分析内源性NO对绿豆侧根发生的作用。【结果】 SNP能显著促进绿豆侧根发生，其中以50 μmol/L SNP处理24 h效果最佳，平均单株生根数为18；IBA或NAA与SNP混合处理的生根效果优于各自单独处理。 L-NAME和c-PTIO单独处理或分别与IBA和NAA混合处理，均能抑制或延缓IBA和NAA诱导的侧根发生。【结论】 内源NO在侧根发生及其形成中可能起着重要作用。 |
| 关键词: 硝普钠（SNP） 一氧化氮 IBA NAA 绿豆 侧根发生 |
| DOI： |
| 分类号: |
| 基金项目:陕西省自然科学研究计划项目（99SM20） |
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| Effect of nitric oxide on the lateral root’s generation of mung bean |
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DUAN Li jun1 2 CAO You long2 ZHOU Jun3
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| Abstract: |
| 【Objective】 Effect of nitric oxide(NO)on lateral rooting of mung bean was investigated.【Method】 With mung bean as study material,the radicles were treated with sodicem nitroprusside(SNP),c-PTIO of NO specific scavenger and NOS inhibitor L-NAME alone or plus IBA or NAA to analyze the effect of endogenous NO on lateral rooting of mung bean.【Result】 The results showed that SNP could significantly enhance the lateral rooting and the effects of IBA or NAA in stimulating rooting,50 μmol/L SNP for 24 h being the best,average lateral roots per plant 18.c-PTIO of NO specific scavenger,or NOS inhibitor L-NAME treated alone or plus IBA or NAA resulted in an inhibitory effect.【Conclusion】 It’s indicated that endogenous NO appears to play a key role in the generation and development of lateral roots. |
| Key words: sodium nitroprusside nitric oxide IBA NAA mung bean lateral root generation |
