| 摘要: |
| [目的]研究苦马豆素(Swainsonine, SW)降解酶的酶促降解特性.[方法]应用渗透休克原理,提取SW降解菌株YLZZ-1粗酶液,进行酶促降解研究,并考察SW降解酶的酶活力、动力学参数及稳定性.[结果]YLZZ-1菌株的胞内粗酶液对SW的降解率高达91.73%.SW降解酶对SW酶促降解的最适pH为8.0,最适温度为30℃,在pH6.0～8.5、温度25～45℃具有较好的降解活性,其米氏常数Km为0.073 98 mmol/L,最大反应速度Vmax为0.111 5 μmol/(mg·min).SW降解酶有较好的pH稳定性,但对热不稳定.[结论]菌株YLZZ-1的SW降解酶属于胞内诱导酶,具有良好的降解能力. |
| 关键词: 苦马豆素 生物降解 不动杆菌 酶促降解 疯草 |
| DOI： |
| 分类号:S859.8 |
| 基金项目:国家自然科学基金? |
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| Enzymatic Degradation Characterization of Swainsonine Degrading Bacterium Strain |
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ZHAO Xing-hua HE Xin WANG Jian-na WANG Jian-hua GENG Guo-xia
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| Abstract: |
| 【Objective】 Degradation characteristics of swainsonine by the crude enzyme of strain YLZZ 1was investigated.【Method】 Crude enzyme of YLZZ-1 was extracted through osmotic shock principle.The enzyme activity,dynamic parameter and stability of the main catabolic enzymes were researched.【Result】 The degradation rate for SW by intracellular enzyme was 91.73%.The pH optimum for crude enzyme was 8.0 for enzymatic degradation of SW,and it had comparatively high activity in the range of pH 6.0-8.5.The optimum temperature for enzymatic activity was 30 ℃,and it still had comparatively high activity in the range of temperature 25-45 ℃.The crude enzyme showingKm value was SW 0.073 98 mmol/L，and the maximal enzymatic degradation rate was 0.1115 μmoL/(mgomin).The enzyme had the stable pH,but was sensitive to high temperature.【Conclusion】 The intracellular and induced enzyme was the main SW catabolic enzymes of YLZZ-1,and it had excellent degradation ability. |
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