| 摘要: |
| [目的]探讨新城疫病毒(Newcastle disease virus, NDV)磷蛋白基因(P基因)的分子进化特点,为科学防控新城疫(Newcastle disease,ND)提供依据.[方法]对1997～2007年国内分离的13个NDV毒株的P基因进行扩增测序,与15个已发表的国内外不同时期的NDV毒株P基因进行核苷酸和推导的氨基酸遗传变异分析及分子特性研究.[结果]国内NDV分离株P基因核苷酸和P蛋白氨基酸高度同源,同源性分别为93.9%～99.8%和93.4%～99.2%.国内NDV毒株与LaSota、Clone30和F48E9等P基因核苷酸和P蛋白氨基酸同源性分别为82.2%～87.7%,81.3%～83.8%;与国外毒株则分别为82.1%～90.2%,81.1%～90.7%.氨基酸序列分析表明,我国毒株具有独特的氨基酸位点.分子进化分析表明,NDV P基因中核苷酸同义替代率高于非同义替代率.[结论]国内分离NDV毒株P蛋白遗传距离较近,而与LaSota、Clone30、F48E9以及国外毒株遗传距离较远,有一定的地域性.NDV P基因以净化选择的方式进化. |
| 关键词: 新城疫病毒 P蛋白 P基因 |
| DOI： |
| 分类号:S852.659.5 |
| 基金项目:山东省科技攻关项目? |
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| Genetic characteristics and evolution of phosphoprotein gene of the field Newcastle disease virus |
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LIANG Jun-wen TIAN Fu-lin HUANG Bing LAN zhou-ran SUN Sheng-fu ZHUANG Wen-zhong
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| Abstract: |
| 【Objective】 To scientifically control and prevent Newcastle disease (ND),this study researched the molecular evolution characteristics of Phosphoprotein(P) gene of Newcastle disease viruses (NDV).【Method】 The nucleotide sequences and deduced amino acid sequences were analyzed for P gene of thirteen field isolates of NDV during 1997-2007 in China.The P gene sequences of fifteen NDV reference strains in GenBank were also used in this study.【Result】 The nucleotide and amino acid homology of Chinese field NDV strains was 93.9%-99.8% and 93.4%-99.2% respectively,but 82.2%-87.7% and 81.3%-83.8% compared with LaSota,Clone30 and F48E9,and 82.1%-90.2% and 81.1%-90.7% to foreign NDV strains.The unique residues in the amino acid sequence were found in the Chinese isolates.Synonymous nucleotide substitutions were more than non-synonymous substitutions.【Conclusion】 There was the nearest distance,compared with the LaSota,Clone30 and F48E9, and the character of geographical areas among Chinese NDV isolates. The evolution of NDV P gene underwent purifying selection. |
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