| 摘要: |
| [目的]粗山羊草是小麦野生近缘属种,是D基因组的供体,蕴含大量的抗病资源,是进行小麦遗传改良的重要遗传资源,明确其抗病基因的数量、类型、在染色体上的位置以及其与已知抗条锈病基因间的关系,挖掘抗条锈病新基因.为小麦育种提供优良抗病新种质.[方法]用离体叶和田间鉴定方法鉴别来自不同产地的38份粗山羊草的抗条锈病情况,对条锈病抗病性进行遗传分析,并利用SSR分子标记定位粗山羊草中的抗病基因.[结果]离体叶鉴定发现,有9份材料对条中29和条中31菌株免疫,占供试材料的23.68%;有6份材料高感或中感,占供试材料的15.79%,其余材料抗病等级不一致.田间混合菌种鉴定结果表明,有19份材料免疫,其中10份材料苗期感病但成株期抗病,占供试材料的26.32%.从粗山羊草(Aegilops tauschii(Coss.)Schmal)Y212中鉴定出1个显性抗小麦条锈病基因,暂定名为YrY212.应用分离群体分组法(BSA)筛选到Wmc506、Barc184,Wmc450和Cfd41标记,其与YrY212之间的遗传距离分别为3.0,4.0,7.0和20.0 cM,位于Wmc506和Barc184之间.[结论]根据连锁标记所在小麦微卫星罔谱的位置,YrY212被定位在7DS染色体上,分析基因所在染色体的位置、抗病性特征认为,YrY212是一个新的抗小麦条锈病基因. |
| 关键词: 粗山羊草 小麦条锈病 抗病基因 基因定位 SSR标记 |
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| 基金项目:河北省科学技术研究与发展指导计划项目“微卫星标记小麦野生资源抗病基因及抗病基因的利用”（05225510）；河北省教育厅科学研究计划项目“微卫星标记小麦抗白粉病基因” （Z2006103） |
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| Identification and SSR mapping of stripe rust resistance gene in Ae. Tauschii |
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| Abstract: |
| 【Objective】 As the wild relatives of wheat and the D genome donor of wheat,the Aegilops tauschii has many resistant resources,and it is an important genetic resource in wheat breeding.【Method】 Thirty-eight specimens of Aegilops tauschii originated from different regions by inoculation of fragments of leaves in vitro were identified,and in field with mixed physiological method the stripe rust resistance gene was socated in Ae.tauschii with SSR marker.【Result】 The results were detested with 2 physiological race of wheat stripe rust （tiaozhong 29 &31）by inoculation of fragments of leaves in vitro（detached leaves）.Nine of them were found immunity to all of wheat stripe rust,five were susceptible to both of the two physiological race.The results from inoculation on seedling and adult plants in field with mixed physiological race were similar to that from the above mothed,just as below,nineteen of them were immunity to wheat stripe rust (tiaozhong 29 &31),ten of them were susceptible at seedling and resistance at adult stage to wheat stripe rust.A dominant wheat stripe rust resistance gene,temporarily designated as YrY212,was identified in an accession Y212 of Aegilops tauschii.By bulk segregation analysis,four microsatellite markers Wmc506,Barc184,Wmc450 and Cfd41 were found to be linked to YrY212 with genetic distances of 3.0,4.0,7.0 and 20.0 cM,respectively.【Conclusion】 According to the locations of the linked markers,the resistance gene was located on chromosome 7DS.Based on the chromosomal location and the resistance pattern of the gene,YrY212 is proposed to be a novel stripe rust resistance gene. |
| Key words: Aegilops tauschii wheat stripe rust resistance gene gene location SSR marker |
