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1-甲基环丙烯对猕猴桃贮藏品质和蛋白质组分的影响
唐 燕1, 杜光源1, 马书尚1
西北农林科技大学 生命科学学院
摘要:
[目的]研究了室温贮藏条件下1-甲基环丙烯(1-MCP)对猕猴桃果实呼吸、乙烯释放、贮藏品质和蛋白质组分变化的影响,为研究1一MCP在猕猴桃商业贮藏中的应用及1-MCP的作用机理提供理论依据.[方法]以‘秦美'和‘海沃德'猕猴桃为试材,研究了1-MCP在室温((20±1)℃)贮藏条件下对果实呼吸速率、乙烯释放速率、果实硬度、可滴定酸含量、可溶性同形物含量及可溶性总蛋白组分的影响.[结果]0.1μL/L 1-MCP处理能降低猕猴桃果实的呼吸速率和乙烯释放速率,抑制果实软化,延缓可滴定酸含量的下降,增加可溶性固形物含量;随着贮藏时间的延长,有特异蛋白条带出现,1-MCP处理对‘秦美'猕猴桃19.0 ku特异蛋白条带有抑制作用.[结论]1-MCP处理能延缓猕猴桃果实的衰老进程.两个猕猴桃品种相比,‘海沃德,猕猴桃产生的乙烯远低于‘秦美'猕猴桃,乙烯高峰出现也晚,果实的软化进程也较‘秦美'猕猴桃慢.
关键词:  1-MCP  贮藏品质  猕猴桃  呼吸速率  乙烯  蛋白蛋组分
DOI:
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基金项目:美国罗门哈斯中国公司资助项目
Effects of 1-rnethylcyclopropene on storage quality and protein composition in kiwifruit
Abstract:
【Objective】 The purpose of the paper is to investigate the effects of 1-methylcyclopropene on storage quality and protein composition in kiwifruit.【Method】 ‘Qinmei’and Hayward’kiwifruits treated with 0.1 μL/L 1-methylcyclopropene (1-MCP) were stored at room temperature((20±1) ℃).The effects of 1-MCP on respiration rates,C2H4 production,flesh firmness,titratable acid,total soluble solids,as well as the change in protein composition were investigated during storage.【Result】 1-MCP inhibited significantly respiration rates and C2H4 production of stored‘Qinmei’and Hayward’kiwifruits.1-MCP significantly slowed down flesh softening and the reduction of titratable acid.Compared with‘Qinmei’,the respiration and ethylene production rate of‘Hayward’kiwifruit were far lower at room temperature,producing much less C2H4 and the peaks of C2H4 appeared later.SDS-PAGE analysis indicated that a 19.0 ku protein band appeared in‘Qinmei’ kiwifruit after 3 week storage and 1-MCP inhibited the appearance of this band.【Conclusion】 The senescence process of kiwifruits was delayed by 1-MCP treatment.Compared with‘Qinmei’ kiwifruit,‘Hayward’ produced much less C2H4 and the peaks of C2H4 appeared later.This might result in slower softening of ‘Hayward’ kiwifruit.
Key words:  1-MCP  storage quality  kiwifruit  respiration  ethylene  protein composition