| 摘要: |
| [目的]研究不同春石斛兰品种组培繁殖能力的差异及6-BA质量浓度对春石斛兰增殖系数的影响,为优化春石斛兰培养基配方和工厂化生产提供技术依据.[方法]以23个引自日本的春石斛兰品种为材料,在诱导培养基(1/2 MS NAA 1.0 mg/L 6-BA 4.0 mg/L卡拉胶7.0 g/L 蔗糖20 g/L 椰子乳100 mL/L 细菌学蛋白胨2.0 g/L)和相同培养条件下,运用SAS软件分析了23个春石斛兰品种间扁动率、增殖率和增殖系数3个指标的差异性;在此基础上,选出增殖系数高、中、低的3个代表品种022,021和026,利用Excel分析了增殖系数与6-BA质量浓度的关系.[结果]在相同诱导培养基和培养条件下,23个春石斛兰品种间的启动率和增殖率差异显著,增殖系数差异极显著;在观察的483个外植体中,平均增殖系数为1.89,低于2.0的观测值占总数的51.97%;3个代表品种021,022和026的拟合曲线表明,其增殖系数随6-BA质量浓度的增加呈现先增后减的变化趋势,增殖系数取最大值时的6-BA质量浓度分别为2.07,3.39和1.92 mg/L.[结论]不同品种春石斛兰的组培增殖能力差异较大,001,002,004和022等4个品种适合在1/2 MS NAA 1.0 mg/L 6-BA 4.0 mg/L 卡拉胶7.0 g/L 蔗糖20 g/L 椰子乳100 mL/L 细菌学蛋白胨2.0 g/L诱导培养基上实现快速繁殖,适合春石斛兰组培增殖的有效6-BA质量浓度为1.9～3.4 mg/L. |
| 关键词: 春石斛兰 增殖系数 启动率 增殖率 |
| DOI： |
| 分类号: |
| 基金项目:广东省科技攻关项目 (2004B50201004,2003A2010401,2006A20201001) |
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| Differences of propagation coefficient in 23 nobile-type Dendrobium varieties introduced from Japan |
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| Abstract: |
| 【Objective】 The propagation ability differences of different variety nobile-type Dendrobium and the effect of 6-BA concentration on their propagation coefficient during tissue culture were studied in order to offer technical basis for their large-scale production.【Method】 The bud tips of 23 varieties nobile-type Dendrobium introduced from Japan were incubated to the induction medium:1/2 MS+NAA 1.0 mg/L＋6-BA 4.0 mg/L+carrageenan 7.0 g/L＋sucrose 20 g/L+coconut water 100 mL/L＋bacterial peptone 2.0 g/L,the percent germination of 23 varieties were compiled after 30 d culture under dark condition.They were subcultured 30 d once,percent propagation and propagation coefficient were compiled after the second subculture,the differences of percent germination,percent propagation and propagation coefficient among 23 varieties were analyzed by SAS statistical software;On the basis of above results,the three representative varieties 022,021 and 026,which had the maximum propagation coefficient,were centered and the minimum value were selected.Their bud tips were incubated to the inducing medium supplemented with NAA 0.5 mg/L and 6-BA 0.5，1.0，2.0，3.0，4.0，5.0 and 6.0 mg/L respectively,their propagation coefficients were compiled after 30 d dark culture and one subculture,their quadratic regression equations associated with the relationship between propagation coefficient and 6-BA qualitative concentration were obtained by software Excel.【Result】 The differences of percent germination and percent propagation among 23 varieties nobile-type Dendrobium were significant.The differences of propagation coefficient were extremely prominent,while they were cultured on the same inducing medium,among the 483 observed explants,their mean propagation coefficient equaled to 1.89,the percent of observation value below 2.0 was 51.97%;The regression equations of three representative varieties 021,022 and 026,showed that their propagation coefficients had the tendency to increase first and decrease then,associated with the 6 BA qualitative concentration increase,and obtained the 6-BA qualitative concentration that were the solutions of the regression equation when their propagation coefficient reached maximum,2.07,3.39 and 1.92 mg/L respectively.【Conclusion】 The differences of tissue culture propagation ability in different varieties of nobile-type Dendrobium were more distinct.The induction medium was suitable for rapid propagation of the four varieties,001,002,004 and 022,the margin 6-BA qualitative concentrations of the three representative varieties demonstrated that the available 6-BA qualitative concentration range suit for tissue culture propagation of nobile-type Dendrobium was 1.9-3.4 mg/L. |
| Key words: nobile-type Dendrobium propagation coefficient percent germination percent propagation |
