| 摘要: |
| [目的]从辣椒花药中克隆与胚状体发育相关的基因,并对其进行序列分析.[方法]以LTP基因、GST基因同源序列设计简并引物,采用RT-PCR方法对辣椒小孢子胚状体发育相关基因进行克隆.测序后对其进行分析.[结果]获得2个可能与辣椒花药胚状体发育相关的基因片段PELTP(GenBank登录号为:EF583618)和PEGST(GenBank登录号为:EF583619),其长度分别约为750 bp和1 000 bp.序列分析表明,PELTP基因与辣椒LTP基因同源性为98%,PEGST基因与辣椒GST基因的同源性为99%。[结论]PELTP和PEGST基因可能在辣椒小孢子胚状体发育早期起着重要作用。 |
| 关键词: 辣椒 花药培养 胚状体 基因克隆 |
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| 基金项目:国家自然科学基金项目（30771467）；国家“十一五”科技支撑计划项目(2006BAD01A7)；西北农林科技大学植物育种专项(05YZ024-1) |
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| Cloning and analysis of embryogenesis related gene in pepper anther culture |
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| Abstract: |
| 【Objective】 The objective of this study was to clone embryogenesis related gene from pepper anther culture and cnalyze their sequences.【Method】 Homologous sequence amplification method was used in gene isolation.The RT-PCR method was used to identify differential gene expression in the process of microspore-derived embryogenesis in pepper.【Result】Two gene sequences PELTP(accession number:EF583618) and PEGST(accession number:EF583619) were obtained,which proved to be expressed at the embryoids.The sequence lengths were 750 bp and 1000 bp respectively.Blast results showed that PELTP was highly homologous to LTP gene of pepper and PEGST to GST gene of pepper.Homology was 98% and 99% respectively.【Conclusion】 It is presumed that the two genes may play an important role in the early stages of pepper microspore embryogenesis. |
| Key words: pepper(Capsicum annuum L) anther culture embryoid gene cloning |
