| 摘要: |
| [目的]优化鸡腿蘑菌丝体水溶性粗多糖(CMP)的提取条件,对鸡腿蘑菌丝体胞内水溶性CMP进行分级分离.[方法]以多糖得率为测定指标,选取提取温度、提取时间、料水比、微波处理时间4个影响因素,通过L9(34)正交试验优化鸡腿蘑菌丝体水溶性粗多糖的提取条件,并通过DEAE-纤维素32柱层析和Sephadex G-200凝胶过滤色谱,对经过初步提纯的鸡腿蘑菌丝体胞内水溶性CMP进行分级分离.[结果]鸡腿蘑菌丝体水溶性CMP适宜的提取条件为:湿菌丝体匀浆后微波处理3 min,然后按料水体积比1:2加水,在55℃水浴中抽提6 h,提取液用无水乙醇醇析,该法CMP的得率为5.14%.DEAE-纤维素32柱层析得到了CMP-Ⅰ、CMP-Ⅱ和CMP-Ⅲ 3个多糖组分,其中CMP-Ⅰ为主要组分,约占69.72%.CMP-Ⅰ含有相对分子质量十分接近的2个组分CMP-Ⅰ-1和CMP-Ⅰ-2,其中CMP-Ⅰ-1为主要组分.[结论]初步确定了腿蘑菌丝体水溶性CMP的提取条件和分级分离方法,为进一步进行CMP结构分析奠定了基础. |
| 关键词: 鸡腿蘑 菌丝体 水溶性多糖 |
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| Extraction and Isolation of Coprinus comatus mycelial water-soluble polysaccharide |
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| Abstract: |
| 【Objective】 The study was to optimize the extractive conditions of Coprinus comatus mycelial water-soluble polysaccharide,grade and isolate Coprinus comatus mycelial water-soluble polysaccharide.【Method】 Used mycelial water-soluble polysaccharide as index,by L9(34) orthogonal test of four factors (tempreture,time,mycelia/water,microwave heating time),the extractive conditions of mycelial water-soluble polysaccharide were optimized.The partial purified polysaccharides were further graded and isolated by DEAE cellulose32 colume and Sephadex G-200 column chromatography. 【Result】 The extractive conditions of mycelial water-soluble polysaccharide were determined as follows:mycelia∶water=1∶2,microwave heating time 3 minutes,extracting temperature 55 ℃,extracting time 6h.The extractive efficiency of mycelial polysaccharide was 5.14%.Three factions in CMP were obtained by DEAE cellulose32 colume, respectively.CMP-Ⅰwas the main faction,accounting for 69.72% of total amount.Then CMP-Ⅰwas further graded and isolated by Sephadex G-200 column chromatography.The result showed that the molecular weight of the two factions were similar which obtained in CMP-Ⅰ,respectively.GMP-Ⅰ-1 was a main faction. 【Conclusion】 The extractive conditions and isolation method of Coprinus comatus mycelial water-soluble polysaccharide were probed. |
| Key words: Coprinus comatus mycelia water-soluble polysaccharide extraction isolation |
