| 摘要: |
| [目的]研究病原菌侵染后不同抗感品种汉中红皮蒜和苏联改良蒜防御酶体系的变化.[方法]每个大蒜品种取生长一致的蒜苗30株人工接种病菌,待植株出现明显症状后,从发病植株和未发病植株的生长点处,采集幼嫩组织测定防御酶活性的变化.[结果]当白腐病菌侵染大蒜后,激活寄主体内相关防御酶系,使苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和多酚氧化酶(PPO)4种酶活性明显提高,抗病品种汉中红皮蒜的酶活性升高幅度大于感病品种苏联改良蒜;而过氧化氢酶(CAT)活性下降,抗病品种汉中红皮蒜的酶活性下降幅度低于感病品种苏联改良蒜.[结论]防御酶活性高低与大蒜品种的抗性关系密切. |
| 关键词: 大蒜 白腐病菌 寄主 防御酶系 |
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| 基金项目:中德农业部科技合作项目(06-37);国家“十五”科技攻关项目（2004BA516A09） |
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| Effect of white rot pathology (Sclerotium cepivorum Berk.) on defensive enzymes of garlic |
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| Abstract: |
| 【Objective】 The experiment aimed at making clear the changes of defensive enzymes after inoculation by Sclerotium cepivorum Berk.in two different resistant cultivars.【Method】 Thirty garlic seedlings were inoculated with germs.After obvious symptoms appeared,the changes of defensive enzymes were determined in health and sick plants.【Result】 The inoculation of pathology could activate the activities of defensive enzymes in garlic.The results suggested that activities of PAL,SOD,POD and PPO were increased after inoculating regardless of in resistant cultivars or susceptible cultivars,and the enzyme activities in resistant cultivar Hanzhong Red Skin were higher than those of susceptible cultivar Gailiang.However,the activities of CAT was lower after inoculating regardless of resistant cultivars or susceptible cultivars,and the activities of CAT in susceptible cultivar dropped more than that in resistant cultivar.Resistance of the different garlic cultivars was significantly correlated with the defensive enzymes.【Conculison】 The activity of defensive enzymes is closly related with the resistance of cultivars. |
| Key words: garlic Sclerotium cepivorum Berk. host defensive enzymes |
