| 摘要: |
| [目的]探索供试生防放线菌Actl、Act8和Actl1与生防真菌C、D、M1和M2混合接种对辣椒根系生长、生防菌定殖能力及辣椒叶片和根系诱导抗性的影响.[方法]采用皿内拮抗试验确定适宜的生防真菌与生防放线菌组合,以甜椒和线椒幼苗为供试材料,蘸根接种处理后,于30和31 d时测定生防菌在辣椒根部的定殖量及辣椒叶片和根系的多酚氧化酶(PPO)活性,研究不同接种处理对生防菌在辣椒根部定殖能力及辣椒叶片和根系PPO活性的影响.[结果](1)4株生防真菌与3株拮抗性生防放线菌形成的12个组合中有7个组合混接菌种之间无相互拮抗作用.(2)生防菌混合接种时甜椒叶片PPO活性的增率大于生防菌单独接种,混和接种处理的最大接种增率约为单接处理的5倍.(3)生防菌单独接种可使甜椒根系PPO酶活异常升高,但生防菌混接(除Actl+C、Actl1+C、Actl1+M1和Actl1+M2外)会导致甜椒根系PPO活性降低.(4)Actl与生防真菌C、M1、M2混接和Act8与D、M1、M2混接均可明显促进甜椒根系生长.(5)在混接处理中,供试生防真菌不能促进生防放线菌在甜椒根内定殖,但MI可促进生防放线菌在甜椒根表的定殖;供试生防放线菌均能促进真菌M2在甜椒根内的定殖.[结论]生防真菌与生防放线菌混合接种(除Act8+D、Actl1+D外)对辣椒叶片PPO活性的提高幅度大于单独接种,混合接种(除Actl1+C、Actl1+M1、Actl1+M2外)对根系PPO活性的降低幅度大于单独接种;混合接种不能促进生防放线菌在辣椒根内定殖,但能显著促进根系生长. |
| 关键词: 生物防治 辣椒疫病 植物免疫 多酚氧化酶 放线菌 混合接种 |
| DOI： |
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| 基金项目:陕西省科技攻关专项“生物农药研究与产业化”（2003K03-G2-04） |
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| Effect of the inoculation with the fungi and actinomycetes against Phytophthora capsici and the colonization capability and the growth and PPO activity of capsicum's leaf and root |
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| Abstract: |
| 【Objective】 This study is to research the associative inoculation's effects on colonization capability and promoting the growth and PPO activity of Capsicum.【Method】 Biocontrol fungi and actinomycetes compounding group were selected after the antimicrobial experiment on the antimicrobial actinomycetes against the fungi and general mensuration of PPO activity.The fungi and actinomycetes associative inoculation to Capsicum were applied by dipping root method then the colonization capability and the Capsicum leaf and root PPO activities were measured.【Result】 (1) Four strains of biocontrol fungi and three strains of antimicrobial actinomycetes were selected.There are 12 associative inoculation groups.In 7 groups,there are no effects between fungi and actinomycetes.(2) The associative inoculation of biological control fungi and actinomycetes can increase the PPO activity of pimiento's leaf and better than individual inoculation.The highest rate is nearly 5 times than individual inoculation.(3) Except Act1+C,Act11+C,Act11+M1 and Act11+M2，overrun inoculation can abnormally increase the PPO activities;associative inoculation can reduce the PPO activity of pimiento's root.(4) Associative inoculation （Act1 with D,M1 and M2,Act8 with these 4 kind of fungi）can increase the weight of pimiento's root prominently.(5) Associative inoculation can not promote the colonization of actinomycetes in pimiento's root,only M1 can help the colonization of actinomycetes on pimiento's root.The biocontrol actinomycetes can increase the colonization densities of M2 in pimiento's root.【Conclusion】 The associative inoculation of biocontrol fungi and antimicrobial actinomycetes (except Act8+D,Act11+D) can increase the PPO activities of pimiento's leaves,associative inoculation (except Act11+C,Act11+M1,Act11+M2) reduce the PPO activities of pimiento's roots after individual inoculation of biological control fungi and antimicrobial actinomycetes,can not help actinomycetes colonized in pimiento's root,but can promote the root growth prominently. |
| Key words: biocontrol Phytophthora capsici Leaonian phyto-immunology PPO actinomycetes associative inoculation |
