| 摘要: |
| [目的]探讨接种生防放线菌对西瓜根域微生物区系的影响.[方法]采用营养钵育苗基质拌菌法接种,进行盆栽和小区种植,通过稀释平板涂抹法测定西瓜根区、根表土壤及根内的细菌、真菌和放线菌数量,并对优势细菌和真菌进行了初步鉴定.[结果]生防放线菌Actl接入西瓜育苗基质后,西瓜根域细菌数量较对照提高3.3%～1 263.2%,其中西瓜根表土壤中芽孢杆菌数量较对照增加122.2%～867.7%;放线菌数量提高17.8%～478.4%;真菌数量减少29.2%～45.2%,其中接种5.0 g/kg放线菌制剂处理西瓜根区和根表土壤中镰刀菌数量较对照分别降低55%和60%;细菌/放线菌较对照提高75.4%～369.5%.接种100 d后,西瓜根域土壤中Actl活菌数量仍然高达106g-1以上.[结论]生防放线菌Actl具有较好的定殖稳定性,能够促使西瓜根域土壤由真菌型向细菌型转变,可在西瓜根域形成含有大量拮抗性放线菌、可抗御西瓜枯萎菌等土传病害致病菌的生物屏障. |
| 关键词: 生物防治 西瓜 连作障碍 土传病害 放线菌 微生物区系 微生态 |
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| 基金项目:陕西省科技攻关项目（2004K02-G7）；国家“十一五”科技支撑计划项目（2006BAD05B07） |
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| Adjusted effect of inoculating with biocontrol actinomyces on microbial flora of watermelon rooting zone |
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| Abstract: |
| 【Objective】 The study was to discuss the effect of inoculating with bio-control actinomyces on microbial flora of watermelon rooting zone.【Method】 With pot experiment and plot experiment,inoculating with bio-control actinomyces in nursery bed soil,the quanties of bacteria,fungi and actinomyces were determined via dilution and plating.Some dominative bacteria and fungi were identified preliminarily.【Result】 Inoculating with Act1 in nursery bed soil had raised the number of bacteria and actinomyces in the rooting zone soil of watermelon and reduced fungi the number of.The quantity of bacteria increased by 3.3%-1 263.2%,the quantity of gemma bacillus of watermelon root surface soil increased by 122.2%-867.7%,actinomyces increased by 17.8%-478.4%,fungi decreased by 29.2%-45.2%,thereinto the destiny of Fusarium in the watermelon rhizosphere soil and root surface soil inoculated by 5.0 g/kg preparetion decreased markedly by 55% and 60% respectively.B/F increased by 75.4%-369.5%.100 days after inoculation,the number of Act1 was more than 106 g-1.【Conclusion】 These results demonstrate an expectant colonization stability of Act1.After inoculation with Act1,the microbial flora of watermelon rooting zone soil changed markedly,from fungi type to bacteria type.In watermelon rooting zone soil,a biological barrier formed mostly by bio-control actinomyces against soil borne plant diseases such as watermelon Fusarium wilt. |
| Key words: biological control watermelon continuous cropping obstacle soil borne plant diseases actinomyces microbial flora micro-ecosystem |
