| 摘要: |
| [目的]研究苗床拌土接种生防菌制剂对辣椒根域微生态及产量的影响.[方法]以真菌F、放线菌Act 2和Act 8为供试生防菌,采用苗床拌土法接种辣椒,平皿涂抹法测定各处理辣椒根区、根表土壤和根内微生物数量,小区试验研究接种生防菌对辣椒产量的影响.[结果]苗床拌土接种2株生防放线菌和1株生防真菌制剂:(1)可明显增加辣椒根域土壤中细菌和放线菌数量,减少真菌数量,改变根域土壤中微生物组成,促使辣椒根域土壤微生物类型由真菌型向细菌型转变.其中接种生防真菌F后,小区辣椒根区土壤细菌、放线菌数量较对照分别增加了166.7%和46.2%,细菌、放线菌与真菌的数量比B/F、A/F分别较对照增加了207.7%和72.2%.(2)显著增加辣椒根表土壤芽孢杆菌数量,减少根区土壤芽孢杆菌数量,根表芽孢杆菌数量增幅和根区土壤芽孢杆菌数量降幅分别为7.5%～246.8%和11.9%～74.5%.(3)显著增加了辣椒根内细菌和放线菌数量,其中芽孢杆菌数量增加明显;接种生防放线菌Act 2后,细菌、芽孢杆菌和放线菌数分别增加314.8%,409.1%和77.3%.(4)显著提高了小区辣椒产量,其中接种生防放线菌Act 8后,鲜椒总产量较对照增加了48.8%.苗床接种生防菌后,在辣椒收获期,小区辣椒根区、根表土壤中仍有大量生防放线菌检出(105～106g-1),表明接入的生防菌可在辣椒根区土壤中长时间定殖、存活,持续发挥生防作用.[结论]苗床拌土接种生防菌可显著改变辣椒根域微生物组成和数量,具有明显的促生、增产作用;苗床拌土接种育苗是一种可用于土传病害生物防治的、值得深入研究的接种方法. |
| 关键词: 生物防治 辣椒疫病 苗床接种 根域微生态 生防放线菌 生防真菌 |
| DOI： |
| 分类号: |
| 基金项目:陕西省科技攻关专项（2003K03-G2-04,2004k02-G7） |
|
| Effect of the inoculation with the antagonistic fungi and actinomycetes in seedbed on micro ecological systems of capsicum rooting zone and yield of capsicum |
|
|
| Abstract: |
| 【Objective】 The objective was to research the effect of the inoculation with the antagonistic fungi and actinomycetes in seedbed on micro ecological systems of capsicum rooting zone and yield of capsicum.【Method】 The effect of inoculated with antagonistic microbial on improving the capsicum's growth and increasing the yield was studied by inoculated in the seedbed and plot experiment,and the microorganism numbers in the capsicum rhizosphere,root surface soil and root were determined by Petri dish smearing method.【Result】 Compared with the control,inoculation with one strain of antagonistic fungi and two strains of antagonistic actinomycetes in seedbed:（1）could significantly increase the quantity of the bacteria and the actinomycetes,reduce the fungi number in capsicum rooting zone soil of plot experiment.The microbial flora of capsicum rooting zone soil changed markedly from fungi type to bacteria type.When inoculated with the antagonistic fungi F in the seedbed,the number of the bacteria and actinomycetes in the capsicum rhizosphere soil of plot experiment increased significantly by 166.7％ and 46.2％,the ratio of bacteria to fungi and actinomycetes to fungi increased by 207.7％ and 72.2 ％;（2）could significantly increase the number of Bacillus sp in capsicum rhizosphere soil,and reduce the number of Bacillus sp in capsicum root surface soil.The scope of increase and lessen were 7.5%-246.8% and 11.9%-74.5%;（3）could significantly increase the number of bacteria and actinomycetes in the capsicum root,among them the number of Bacillus sp also increased significantly;when inoculated with Act2,the number of bacteria,Bacillus sp and actinomycetes increased by 314.8%,409.1% and 77.3%;（4）could significantly increase the yield of capsicum in plot experiment.When inoculated with Act8,the yield of capsicum increased by 48.8% compared with the control. Inoculated with the antagonistic fungi and actinomycetes in seedbed,a large amount of antagonistic actinomycetes also been checked in capsicum rhizosphere and root surface soil of the capsicum harvest period（105～106g-1）,which indicated that inoculation with the antagonistic microbial in the seedbed could colonized in the capsicum rhizosphere, and lasts a long time,it could make full use of the micro control strains continuance.【Conclusion】Inoculated with the antagonistic fungi and actinomycetes in the seedbed could significantly change the microbial buildup and quantity,which could make good use of the yield of the capsicum.So inoculation with antagonistic microbial in the seedbed was worth studying for soil borne disease. |
| Key words: biological control Phytophthora capsici Leaonian inoculation in the seedbed microecological systems of capsicum rooting zone antagonistic actinomycete antagonistic fungi |
