| 摘要: |
| [目的]研究苦马豆素-HSA(SW-HSA)对小鼠的免疫原性,为构建SW噬菌体单链抗体库奠定基础.[方法]将12只健康Balb/c小鼠随机分为小剂量免疫组(A组)、大剂量免疫组(B组)和对照组(C组)3组,每组4只.免疫组用SW-HSA加入佐剂后进行了4次免疫,首免和第2次免疫间隔30 d,抗原剂量相同,分别为A组0.05 mg/只,B组0.10 mg/只;第3,4次免疫分别在第50,70天进行,第3次免疫各组抗原量分别是首免的1.5倍,第4次免疫抗原量分别是首免的2.5倍.第3,4次免疫后第10天采血,用间接血凝试验与间接酶联免疫吸附试验检测血清中的SW抗体效价.[结果]第4次免疫后,间接血凝试验检测显示,A、B两组小鼠血清中的抗体效价分别达26和25;间接酶联免疫吸附试验检测显示,A、B两组小鼠血清中的抗体效价分别达29和28.[结论]用SW-HSA免疫Balb/c小鼠,可获得高抗体效价的免疫小鼠. |
| 关键词: 苦马豆素 SW HSA 免疫原性 |
| DOI： |
| 分类号:R383.24 Q78 |
| 基金项目:国家自然科学基金项目（30371067）；西北农林科技大学青年学术骨干支持计划项目（11241） |
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| Study on immunogenicity of swainsonine-HSA in mice |
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YANG Liu YU San-ke FENG Xin-gang YUAN Chun-xiu SHEN Yang LIU Jing-ming LIN Jiao-jiao CAI You-min
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| Abstract: |
| 【Objective】 The immunogenicity of swainsonine-HSA was studied in mice to construct the phage antibody library against swainsoniene.【Method】 12 health male Balb/c mice were divided into three groups.Group A (4 mice) was immunized with low-dose swainsonine-HSA，group B (4 mice) was immunized with high-dose swainsonine-HSA，group C (4 mice) was control.The mice of immune groups were injected four times by SW-HSA emulsified with CFA completely.At the first immunization，each mouse in group A was injected 0.05 mg swainsonine-HSA,0.10 mg swainsonine-HSA for every mouse in group B.The second immunization was directed on the 30th day with SW-HSA emulsified by FIA after the first immunization.The immune doses and ways were same as the first time.The third immunization was done on the 50th day with SW-HSA emulsified by FIA.The immune way was the same as the first time,the dose of group A and group B was 1.5 times of the first immunization.The fourth immunization was done on the 70th day with SW-HSA dissolved by physiological saline,the immune way was subcutaneously of back and peritoneal injection,the dose of group A and group B were 2.5 times of the first immunization.【Result】 The SW antibody titers were assayed by indirect hemagglutination test and enzyme-linked immunosorbentassay on day 10 after the third immunization and fourth immunization respectively.The results showed that，after the 4th immunization，the titers of the antibody against SW assayed by IHA were up to 26 in group A and 25 in group B,the titers of the antibody assayed by ELISA were up to 29 in group A and 28 in group B.【Conclusion】 The mice that were immunized by swainsonine-HSA in the test have obtained high antibody titers. |
| Key words: swainsonine SW-HSA immunogenicity |
