摘要: |
[目的]探讨兔出血症病毒的遗传变异情况,为兔病毒性出血症的防治提供理论资料.[方法]对1989~2006年7株兔病毒性出血症病毒(RHDV)浙江分离株的核衣壳蛋白基因(vP60)进行了克隆与测序,同时利用分子生物学软件将其与国内外RHDV的基因组序列进行了比对和分析,并绘制了系统发生树.[结果]此7株RHDV的VP60基因均由1 740个核苷酸组成,编码580个氨基酸,其与参考序列之间的氨基酸同源性为94.0%~99.8%;系统发生树分析显示,所选的RHDV分离株可分为2个基因群,每个基因群又可分为两个亚群,其中中国分离株主要位于C亚群,与欧洲分离株的遗传距离较近,基因群之间的遗传距离有逐渐加大的趋势.[结论]RHDV在长期流行与演化过程中存在一定的遗传变异趋势. |
关键词: 兔病毒性出血症病毒 衣壳蛋白基因 系统发生树 |
DOI: |
分类号:S858.285.11 |
基金项目:国家自然科学基金项目(30670074); 浙江省自然科学基金项目(Y305047) |
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Sequencing and analyzing of the capsid protein gene of seven rabbit Haemorrhagic disease virus(RHDV) isolated from Zhejiang |
BAI Ting-yang YANG Zeng-qi WU Zhi-ming PU Zhi-ping ZHAO Ming-jun YAN Ruo-qian
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Abstract: |
【Objective】 In order to study the genetic variation of rabbit haemorrhagic disease virus(RHDV),and to provide some useful data to make efficient controlling measures.【Method】 The capsid protein gene(VP60) of seven RHDV isolated from Zhejiang were sequenced.【Result】 The nucleotide sequence of the gene was 1 740nt in length,encoding a protein of 580aa.The VP60 gene of the seven strains shared high sequence homology (from 94.0% to 99.8%) with those of the reference strains;all the RHDV isolates could be divided into two genetic groups (GⅠ and GⅡ),which can be divided into two subgroups(A and B;C and D),respectively.Most of Chinese strains located in C subgroup,and showed closer genetic relation with some European strains.Besides,the phylogenetic tree also indicated that RHDV have evolved into different extents.【Conclusion】 RHDV may exist genetic variation during its epidemic and evolution. |
Key words: Rabbit haemorrhagic disease virus capsid protein gene phylogenetic tree |