| 摘要: |
| 为了探明温度胁迫对不同体色型棉蚜存活状况的影响及其与体内总蛋白含量的关系,对室内饲养的两种体色型棉蚜进行变温（10,15,25和30 ℃）应激处理,研究温度胁迫对棉蚜存活及其总蛋白含量的影响。结果表明,黄色型和绿色型棉蚜仅在处理时间为1 d时可见死亡现象,在10 ℃ 1 d和30 ℃ 1 d时存活率最低,分别为83.33%和51.11%。将活蚜放入各自的对照温度下恢复15 h后,同一胁迫温度下,随着应激时间的延长,棉蚜的存活率开始呈下降趋势,以后慢慢上升,两种体色型棉蚜分别在15 ℃ 1 d和10 ℃ 1 d时存活率最高,达到98.72%和97.48%;且高温有利于黄色型棉蚜生存,低温有利于绿色型棉蚜生存。同一胁迫温度下,随着处理时间的延长,棉蚜体内总蛋白含量呈上升趋势,黄色型和绿色型棉蚜分别在10 ℃ 30 min和30 ℃ 30 min时最大,达到109.99和108.46 μg/mg,超过此时间,棉蚜体内总蛋白含量开始下降,但始终高于各自的对照。棉蚜总蛋白含量随不同时间和温度的变化呈偏峰态趋势,其最佳总蛋白含量的时间和温度区域分别为30～60 min和28～32 ℃及7～12 ℃。 |
| 关键词: 棉蚜 温度胁迫 存活率 总蛋白含量 |
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| 基金项目:国家自然科学基金项目(39970112,30470268);陕西省重点项目(2001SM01) |
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| Effects of temperature on the survival and total proteins content in Aphis gossypii Glover(F.)(Homoptera:Aphididae) |
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| Abstract: |
| In the lab,the survival rate and the total protein content of Aphis gossypii Glover were examined by means of variable temperature.The results were as follows:the death occurred in yellow type and green type only for 1 d treatments,and the survival rates at 10 and 30 ℃ were the lowest,which were 83.33% and 51.11%,respectively.Results suggested that with the stress time prolonged,the survival rates decreased at first,then increased slowly under the same temperature.Furthermore,the higher temperature benefited the yellow Aphids survival and the lower one increased the green Aphids survival.Finally,with the time increasing under the same tempature,the total protein content,showed an increasing tendency.and the values reached the tops at 10 and 30 ℃ for 30 min,which were 109.99 and 108.46 μg/mg.Beyond 30 min the content began to drop down but always above the control.At the meanwhile,there was no significant difference between the two colors Aphids.The total protein content of cotton aphids show the trend of partial kurtosis with the change of different time and temperature,which shows the optimal field of time and temperature on total protein content is 30-60 min and 28-32 ℃ and 7-12 ℃,respectively. |
| Key words: Aphis gossypii Glover temperature stress survival rate total proteins |
