| 摘要: |
| 对37-99 d胎龄的蒙古绵羊胎儿卵巢进行组织学观察。结果表明,绵羊胎儿在37 d,可以从外观上分辨雌雄,雌性胎儿的性腺初步出现卵巢的组织学特征;37-51 d为卵原细胞的共质体期,卵原细胞数量增长加快;55～76 d卵巢中逐渐充满大量的合胞体样卵母细胞群;从58 d开始卵巢皮质深层出现少量的形态不典型的原始卵泡。胎龄73 d时,皮质深层出现真正的原始卵泡;81 d时皮质中层出现大量原始卵泡;99 d时皮质深层中可看到一些初级卵泡。与成体卵母细胞相比,胎儿时期的生殖细胞有活跃的增殖分裂特征;生殖细胞发育按卵原细胞增殖→卵母细胞分化→形成原始卵泡的步骤发育,在位置分布上由卵巢皮质外层逐渐向皮质里层分步发育。 |
| 关键词: 绵羊 胚胎卵巢 卵原细胞 卵母细胞 原始卵泡 |
| DOI： |
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| 基金项目:国家自然科学基金项目（39960051） |
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| The histological study of germ cell genesis in mongolian ovine fetal ovary |
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| Abstract: |
| Using the method of histological routine,histological structure of 37-99 day-old ovine fetal ovaries were studied.When the sheep embryo is 37 days old,it can be distinguished female or male from external appearance.About 37-51 d,the quantity of oogonia grows rapidly and the synplasma-like oogonia can be found in this stage.About 55-76 d,much of the syncytium-like cell group is found in the ovary.The un-typical primordial follicle is found in the ovary cortices from 58 d.The typical primordial follicle is found in deep layer of the ovary cortices from 73 d;and on the 81 d,the number of primordial follicles increases.Primary follicles are found in deep layer of cortices at 99 d.In contrast to mature oocytes,the germ cells are active cell-growing and dividing,changing shape and even migrating within the fetal ovary,hence more changes at this development stage.Our observations provide the morphometric and apoptosis aspects of sheep fetus ovary and evidence that apoptosis in the sheep fetus ovary is associated with germ cells. |
| Key words: mongolian sheep,fetal ovaries,oogonia,oocyte,primordial follicles |
