| 摘要: |
| 采用PCR技术,扩增了猪瘟病毒石门株NS3基因的丝氨酸蛋白酶、NTPase、RNA解螺旋酶活性功能区长度为2 000 bp的片段,将其克隆到pET-32a中,构建了原核表达载体pET-NS3,并将其在大肠杆菌Rosetta(DE3)中进行了表达,同时对表达产物进行了SDS-PAGE和Western blotting检测。结果表明,IPTG诱导表达得到的pET-NS3融合蛋白的相对分子质量约为97 ku,与预期结果一致,且该重组蛋白能被CSFV阳性血清所识别。 |
| 关键词: 猪瘟病毒 NS3基因 大肠杆菌 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(30270988) |
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| Expression of NS3 gene of CSFV Shimen strain in E.coli |
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| Abstract: |
| Serine protease and NTPase/RNA helicase functional domains,2.0 kb of NS3 gene of CSFV Shimen strain were amplified by PCR,and cloned into prokaryotic expression vector pET-32a.The target protein was expressed in E.coli Rosetta(DE3) induced with IPTG,and detected by SDS-PAGE and Western blotting.The results indicated that the 97 ku expressed protein could be recognized by CSFV positive serum as expected. |
| Key words: classical swine fever virus(CSFV),NS3 gene,E.coli |
