| 摘要: |
| 利用改进的DNA提取方法和PCR-RFLP技术，对南阳牛、秦川牛、西镇牛、鲁西牛（地方黄牛品种）和荷斯坦奶牛等5个牛品种的263头个体leptin基因外显子3多态性进行了分析。结果表明，牛leptin基因外显子3长度为330 bp,存在HaeⅢ和却ApaⅠ限制性内切酶的酶切位点，但在这2个酶切位点上无多态性，2种酶酶切片段长度均为70 bp和260 bp。说明牛leptin基因外显子3在HaeⅢ和ApaⅠ2个酶切位点上具有保守性。 |
| 关键词: leptin基因 外显子3 牛品种 多态性分析 |
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| 基金项目:国家自然科学基金项目（30471238）;国家“863”高技术研究与发展计划项目(2003AA243051)；河南省杰出人才创新基金（0521001900）；西北农林科技大学拔尖人才支持计划基金和研究生教育创新基金（05YCH018） |
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| Polymorphic analysis of leptin exon 3 in five Chinese cattle breeds |
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| Abstract: |
| Improved DNA extract method and PCR-RFLP were used in this trial.The polymorphism of leptin exon 3 was analyzed in Nanyang cattle,Qinchuan cattle,Xizhen cattle,Luxi cattle（local yellow cattle breeds） and Holstein cow and 263 individuals were used in this trial.Fragment of 330 bp was amplified with the primer and products were digested with endonuclease HaeⅢ and ApaⅠ,and produced fragments of both were about 70 bp and 260 bp.The results showed that the leptin gene in yellow cattle and Holstein cow had no polymorphism in the two enzymatic digestion sites. |
| Key words: leptin exon 3 PCR-RFLP cattle breed polymorphic analysis |
