| 摘要: |
| 根据GenBank中已公布的人、猪和小鼠的MyoG基因的5’侧翼和部分第一外显子序列设计PCR引物，用touch-downPCR技术扩增了牛MyoG基因的启动子序列,构建了重组克隆载体pGEM-T-MyoG，并通过PCR扩增、限制性酶切对阳性克隆进行了鉴定、测序及生物信息学分析。结果表明，牛MyoG基因的启动子序列（Genbank中注册号为AY882581）长度为672 bp，其与猪、人和小鼠相应序列的同源性分别为94％，91％和88％，且其在不同物种之间具有一定的保守性。牛MyoG基因启动子的克隆与序列分析为进一步研究牛MyoG基因的表达调控奠定了基础。 |
| 关键词: MyoG基因 启动子序列 序列分析 牛 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（30471238）；国家“863”高技术研究与发展计划项目(2003AA243051)；河南省杰出人才创新基金项目（0521001900）；西北农林科技大学拔尖人才支持计划基金项目（01140101） |
|
| Cloning and analysis of the promoter sequence of bovine MyoG gene |
|
|
| Abstract: |
| MyoG gene was investigated to play a core role in regulating myogenisis.According to 5′flank regions and partial sequences of the first exon of MyoG of Homo sapiens,Sos Scrofa and Mus musculus published in GenBank,the homogeneous primers were designed to amplify the promoter region of MyoGof Bos taurus by performing touch-down PCR.The PCR product was ligated to pGEM-T-easy vector,and then E.coli DH5α strain was transformed.Positive clones were identified by restriction endonuclease,PCR and sequencing.The promoter sequence of MyoG of Bos taurus was identified(GenBank Accession No AY882581),which showed 94%，91% and 88% homology with that of Sos Scrofa,Homo sapiens and Mus musculus respectively.It is indicated that the promoter sequence of MyoG was comparatively conservative amongdifferent species.Cloning and analyzing of the promoter region of MyoG made substantial basis for further research on the mechanism of regulation and expression of MyoG in Bos taurus. |
| Key words: MyoG gene promoter sequence sequence analysis Bos taurus |
