| 摘要: |
| 根据牛病毒性腹泻病毒（BVDV）NADL株的序列设计并合成1对引物，对从吉林不同地区分离的4株梅花鹿源BVDV（CCSYD株、CCJYD株、CCKCD株和JLCYD株）的NS2-3基因进行RT-PCR扩增。并对扩增的片段进行序列分析。结果表明，国内梅花鹿源BVDV CCSYD株属基因1b弧型，CCJYD株、CCKCD株和JI，CYD株属基因型待定。 |
| 关键词: 梅花鹿 牛病毒性腹泻病毒 NS2-3基因 序列分析 |
| DOI： |
| 分类号:S852.659.6 |
| 基金项目:国家自然科学基金资助项目（30300256）；吉林省科技发展计划项目（20030552-2） |
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| Cloning and sequencing of NS2-3 of BVDV on sika deer |
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WEN Tie-feng GAO Yu-gang WANG Shu-zhi DU Rui
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| Abstract: |
| A pair of primers were designed and synthesized according to nucleotide sequence of NADL BVDV.Exogenous sequence insertion domain of NS2-3 of CCSYD,CCTYD,CCKCD and JLCYD isolated from different districts of Jilin province was amplified by using RT PCR.The amplified fragments were cloned and sequenced.Results show that CCSYD strain of BVDV in our country belongs to subtype Ⅰb,but CCJYD,CCKCD,JLYD belong to some unknown gene types. |
| Key words: sika deer,bovine viral diarrhea virus,NS2-3 gene,sequence analysis |
