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| 摘要: |
| 从陕西秦岭山区采集的土样中分离到1株具有广谱抗真菌活性的放线菌菌株Z139，经初步鉴定为小多孢菌属红棕褐小多孢菌（Micropolyspora rabrobranea）的一个变种。该菌株发酵液对玉米小斑病菌、苹果轮纹病菌、棉花枯萎病菌、马铃薯干腐病菌和西瓜枯萎病菌菌丝的生长抑制中浓度（EC50）分别为0.979，0.505，1.733，0.425和0.849 mL/L，对玉米大斑病菌、番茄叶霉病菌、马铃薯干腐病菌和烟草赤星病菌的孢子萌发抑制中浓度（EC50）分别为1.709，0.375，0.445和1.333 mL/L。对小麦白粉病的保护和治疗作用分别达到98.6%和86.1%。 |
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| DOI： |
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| 基金项目:国家863高技术计划项目（2002AA245121） |
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| Studies on isolation,identification and fungicidal activity of antagonistic actinomycete Z139 |
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| Abstract: |
| A Actinomycete strain producing a broad-spectrum antifungal substance was isolated from the soil of the Qinling mountain in Shaanxi.The strain numbered Z139 was initially identified as a variety of Micropolyspora rabrobranea.The results of mycelium growth rate showed that the value of EC50 against Bipolaria maydis,Macrophoma kawatsukai,Fusarium oxysporum,Fusarium solani and Fusarium oxysporium were 0.979,0.505,1.733,0.425 and 0.849 mL/L.The result of spore germination proved that the value of EC50 against Alternaria longipes,Exserohilum trucicum,Fulvia fulva and Fusarium solani were 1.709,0.375,0.445 and 1.333 mL/L.The results of pot test indicated that fermention products of Z139 exhibited 98.6% of protected efficacy and 86.1% of therapeutic efficacy. |
| Key words: actinomycetes fungicidal activity Micropolyspora rabrobranea |
