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| 摘要: |
| 利用核糖体内转录间隔区(internal transcribed spacer,ITS)序列通用引物ITS1/ITS4分别获得了大豆疫霉（Phytophthora sojae）和辣椒疫霉（P.capsici）的ITS序列，通过获得序列设计了大豆疫霉的特异性引物PS1和PS2，并建立了分子检测方法。该方法对大豆疫霉(P.sojae)菌丝体和土壤中卵孢子检测的灵敏度分别为10-5 ng/μL和每克土壤0.05个卵孢子。 |
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| 基金项目:国家重点基础研究发展规划(2002CB111406)；国家杰出青年基金项目(30125031)；黑龙江省自然科学基金项目(TC2005-08) |
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| Molecular detection of Phytophthora sojae |
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| Abstract: |
| Genetic PCR (polymerase chain reaction) products from the internal transcribed spacer (ITS) regions of the ribosomal DNA of P.sojae and P.capsici were cloned and sequenced.A pair of primers PS1/SP2 specific for P.sojae were designed according to multiple sequences analysis.PCR based on PS1/SP2 can amplify a 96 bp product from genomic DNA of P.sojae strains,but not other pathogens.PCR with the primers PS1/SP2 detected pathogen at a level of 10-5 ng/μL or 0.05 oospore theoretically.P.sojae in soils and tissues of diseased sobean can also be targeted by the molecular probe.The PCR protocol provides a rapid and reliable tool to detect P.sojae and identify diseases. |
| Key words: Phytophthora sojae ITS analysis PCR molecular detection |
