摘要: |
采用电子显微镜技术研究了苦皮藤素Ⅳ对粘虫(Mythimna separata (Walker))中肠组织的影响。电镜观察结果表明,苦皮藤素Ⅳ对粘虫中肠肠壁细胞的质膜和内膜系统有一定影响。在苦皮藤素Ⅳ作用下,试虫的柱状细胞顶膜微绒毛扩张;线粒体肿胀;粗面内质网扩张,囊泡化;杯状细胞与柱状细胞之间间隙增大。这表明苦皮藤Ⅳ破坏了中肠细胞的结构,使中肠肠壁细胞损伤及细胞间隙增大,导致毒物易于穿过肠壁细胞间隙进入血淋巴,讲而到达作用部位。 |
关键词: 苦皮藤素Ⅳ 粘虫 中肠肠壁细胞 |
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基金项目:国家自然科学基金重点项目(30130130) |
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Ultrastructural effect of Celangulin Ⅳ on the midgut tissue of Mythimna separata larvae |
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Abstract: |
Studies have been conducted with electric microscope on the ultrastructure of midgut on the armyworm larvae (Mythimna separata).The main results are as the followings:Celangulin Ⅳ had some effect on the plasmalemma and internal membrane system of the midgut cell of the armyworm larvae.It was characterized by the increases of the intercellular space between columnar cells and goblet cells with the microvilli of goblet cells dilatated.Endoplasmic reticulum (ER) was expanded with vesiculations appeared.Mitochondria of columnar cells were swollen.It indicated that celangulin Ⅳ had disruptive function on the structure of midgut cells.The increased space of intercellular and the lesion of midgut cells made the toxin easily come through the midgut cell and enter the hemocoel and finally come to the target. |
Key words: Celangulin Ⅳ Mythimna separata Walker midgut cell |