| 摘要: |
| 研究了植物生长调节剂、基本培养基、采样时间对杜仲腋芽诱导和生长的影响，并对丛芽增殖培养基中植物生长调节剂浓度和生根培养基类型进行了筛选。结果表明，最佳取材时间为4月中旬至6月下旬；以MS为基本培养基，在添加0.05 mg/L NAA +0.5 mg/L BA或者0.1 mg/L IBA +0.5 mg/L BA的培养基中，腋芽的诱导和生长较好；继代增殖培养以MS +0.5 mg/L BA +0.01 mg/L NAA的培养基丛芽数量最多，增殖系数最高；在White培养基上生根率可达57.5％。 |
| 关键词: 杜仲 组织培养 快繁技术 |
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| 基金项目:国家林业局948项目(99-4-23)；西北农林科技大学青年科研专项部分内容 |
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| Study on tissue culture micropropagation of Eucommia ulmoides Oliv |
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| Abstract: |
| The influences of axillary bud induction on growth,plant growth regulator's concentration on multiplication of adventitious bud and basic media on root induction through plant growth regulators were studied,and the basic media,sampling times of Eucommia ulmoides Oliv were selected.The results showed that favorite time for explant collection was from the middle ten days of April to the last ten days of June.MS as the basic medium supplemented with 0.05 mg/L NAA+0.5 mg/L BA or 0.1 mg/L IBA+0.5 mg/L BA was beneficial for axillary bud induction and growth.The number of adventitious bud and the rate of multiplication were best on MS supplemented with 0.5 mg/L BA +0.01 mg/L NAA.Rooting rate arrived 57.5% on White medium. |
| Key words: Eucommia ulmoides Oliv. tissue culture micropropagation technology |
