| 摘要: |
| 选取改良蒜、金堂早蒜、苍山蒜和欧引01等4个不同生态型的大蒜品种，采用直接接种和逐级培养法在质量分数0.02％，0.04％，0.06％，0.08％，0.10％，0.20％的草甘磷培养基上，选择抗草甘磷愈伤组织细胞系。结果表明，草甘磷对大蒜外植体的出愈率有极强的抑制作用，在试验中均未诱导出愈伤组织。用愈伤组织直接在不同质量分数的草甘磷培养基上选择，4个品种在低质量分数(＜0.04％)胁迫下都有一定的抗性；经逐级抗草甘磷选择后的愈伤组织，其抗性能力有明显提高，在含有草甘磷质量分数0.06％培养基上，4个品种愈伤组织的成活率为10.3％～18.2％，且发育良好；在质量分数0.08％和0.10％的草甘磷培养基上也有部分愈伤组织成活。抗性稳定性检验证明，此抗草甘磷能力在一定时间内能够保持下去。 |
| 关键词: 大蒜 愈伤组织 变异系 抗草甘磷选择 |
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| 基金项目:中德政府间合作项目(CH-01/04) |
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| Study on in vitro selection for glyphosate-resistant callus of garlic |
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| Abstract: |
| Four different ecotypic garlic cultivars:Gailiang,Jintang early,Cangshan and Europe 01 were chosen for this experiment.Their store-leaf with part of stem plate and callus established from it was used to select glyphosate-resistant on solid MS medium containing gradually increased concentrations of Glyphosate from 0.02%,0.04%,0.06%,0.08% to 0.10%.The results show that Glyphosate inhibited callus growth completely in all tested concentration levels;direct selection on medium containing different concentration of Glyphosate showed that callus of four cultivars all had Glyphosate-resistant at lower concentration (<0.04%) of Glyphosate;Glyphosate-resistant of callus enhanced obviously when selected by step-to-step increasing concentration of Glyphosate from MS medium.The survival rate of callus of four garlic cultivars on medium containing 0.06% glyphosate was in the range of 10.3%-18.2%;Mensuration of growth rate indicates that callus development was good.Some callus were survivor on medium containing 0.08% and 0.10% Glyphosate.Stability test of glyphosate-resistant showed the induced glyphosate-resistant could keep for a long time. |
| Key words: garlic callus variation selection of glyphosate-resistant |
