| 摘要: |
| 采用农杆菌介导的叶盘法，用胡萝卜茄红素-β环化酶反义RNA基因对烟草SRI野生类型种及Xanthi品种进行了遗传转化。GUS检测结果表明，胡萝卜茄红素-β环化酶反义RNA基因对两个不同烟草材料的遗传转化率均为41.7％；Xanthi Southern杂交的阳性率为67％；转基因烟草当代叶片组织中茄红素和胡萝卜素含量与对照植株不同。 |
| 关键词: 烟草 茄红素-β-环化酶反义RNA基因 遗传转化 |
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| 基金项目:国家自然科学基金资助项目(30070525);北京市科技新星项目(9558101400) |
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| Transformation of anti-lyc-b in carrot to tobacco |
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| Abstract: |
| Transformation of anti-lyc-b in carrot to tobacco varieties SRI and Xanthi was done by infecting leaf plates with Agrobacterium tumefaciens carrying expression vector of the gene.GUS test and Southern hybridization revealed that the rates of transformation basing on the GUS test were all 41.7%;Southern hybridization of Xanthi plants positive to GUS test showed the transformation ratio was 67%.Analysis on the contents of lycopene and carotene in Xanthi transgenic plants indicated that there were differences not only between transgenic and non-transgenic plants but also between two different transgenic plants. |
| Key words: tobacco anti-lyc-b transformation |
