| 摘要: |
| 从活性污泥中分离出63株菌株，培养、筛选得到具有絮凝活性的菌株17株，其中2株絮凝活性较高。研究了这2株菌在不同培养时间的生长情况及其絮凝活性等，得出絮凝活性与菌生长量呈正相关关系。通过改变培养基的碳源、氮源、无机盐等因素，得出2株絮凝活性较高的菌株产生絮凝剂的最佳条件：真菌IV-2l，以蔗糖为碳源，蛋白陈为氮源，NaCl为无机盐，pH为6.0，培养温度为30 ℃时，絮凝效果最好；细菌I-9，以葡萄糖为碳源，尿素为氮源，K2HPO4为无机盐，培养基初始PH偏碱性，培养温度为30 ℃时，絮凝效果最好。 |
| 关键词: 絮凝剂产生菌 微生物絮凝剂 絮凝活性 |
| DOI： |
| 分类号: |
| 基金项目:西北农林科技大学重点基金项目 |
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| Screening of flocculant-producing microorganisms and studying on the effective factors |
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| Abstract: |
| 63 strains were isolated from activated sludge.Each strains which was picked out was cultured in relevant flocculant producing liquid mediums.17 flocculant-producing strains were obtained.Among them,two strains with high flocculation activity.In this paper,time course of flocculants production including growth,flocculating activity etc were studied.The best flocculant-producing conditions of the two strains were found by changing medium of carbon sources,nitrogen sources,inorganic salt ion etc.The favorable substrate for fungus Ⅳ-9 were sucrose,peptone,NaCl.Optimum culture initial pH and optimum culture temperature were 6.0 and 30 ℃ respectively.The favorable substrates for bacteria Ⅰ-9 were fructose,urea,K2HPO4.Optimum culture initial pH and optimum culture temperature were 8.0 and 30 ℃. |
| Key words: Flocculant-producing microorganisms microbial flocculants flocculating activity |
