摘要: |
以HgCl2作为水通道蛋白的抑制剂,采用根压液流法测定干旱、未干旱胁迫下西红柿的根系水导速率和根系水分介导常数(Lp)。结果表明,用50,100 μmol/L HgCl2处理根系,未干旱胁迫株根系水导速率分别较对照下降了46.7%和65.67%,用500,1 000 μmol/L ME处理,可分别恢复到对照的88.6%和80.59%;同样条件下干旱胁迫株根系,水导速率分别较对照下降了40.67%和58.82%,并相应恢复到对照的72.03%和79.41%;并且干旱胁迫株根系在0.1~0.3 MPa液压时,根系压力与水导速率(Jv)值呈明显的线性关系,暗示着干旱胁迫株根系存在控制水分运输的水通道蛋白。 |
关键词: 西红柿 根系 干旱胁迫 水导速率 水通道蛋白 |
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Comparison research on water transportation of non-drought and drought-stressed tomato root systems |
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Abstract: |
A pressure-flux approach was used to detect rate of water transportation of non-drought and drought-stressed tomato root systems,when HgCl2 is served as aquaporins inhibitor.The results indicated that rate of water transportation of non-drought roots deceased respectively by 46.7%,65.67% when treated with 50,100 μmol/L HgCl2,and recovery to 88.6%,80.59% when treated with 500,1 000 μmol/L ME.Under same conditions,rate of water transportation of drought-treated root deceased by 40.67%,72.03% correspondently when treated with 50,100 μmol/L HgCl2,and recovery to 72.03%,79.41% when treated with 500,1 000 μmol/L ME.And drought-stressed roots of Jv have obvious linear relations between 0.1 and 0.3 Mpa.All these data and phenomena above indicated that aquaporins exists in drought-stressed roots and mediated water transportation. |
Key words: tomato root systems drought-stressed rate of root water transportation aquaporins |