| 摘要: |
| 在绝食、强饲无氮日粮（NFD）、CP20％（豆粕组）、CP20％（棉粕组）4种处理下，给蛋青鸡每公斤体重静脉一次注射1 110 MBq[3H]Leu，分别于注射后5 min,30 min,4 h,24 h,36 h,48 h翅静脉采血，测定各时间点去蛋白血浆游离Leu浓度和比放射性活度。结果表明，去蛋白血浆中游离Leu浓度绝食组和NFD组显著低于豆粕组和棉粕组（P＜0.05）；血浆游离Leu的比放射性活度（SRp）,以NFD组与绝食组相近，且较高，棉粕组最低，但4种处理间无显著差异（P＞0.05）。血浆游离Leu比放射性活度呈显著的指数衰变规律（P＜0.05）。 |
| 关键词: 血浆游离Leu 比放射性活度 蛋青鸡 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目(39970550) |
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| Change regularity of special radioactivities of plasma free Leucine under four different treatments |
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| Abstract: |
| Twenty-four young hens(1.5 kg of body weight,BW)were randomly divded into 4 groups.Every group was diet free(FAS) or force-fed Abstract: nitrogen-free diet (NFD) or the diet with 20%crude protein in which soybean meal or cottonseed meal was the sole nitrogen source (30 g DM/kg BW).1 100 MBq 3H-Leucine/kg BW wasintravenously injected into all birds just after force-fed or on-fasting.Venous blood samples were taken at 5 min, 30 min ,4 h,24 h,36 h,and 48 h after injection.Special radioactivities of non-protein plasma at every timepoint are measured.The study showed that concentrations of free Leucine in plasma of FAS and NFD were significantly lower than that of CP 20%(soybean meal)and CP 20%(cottonseed meal)(P<0.05).Specific radioactivities of free Leucine in plasma in NFD and FAS were similar and higher and that of CP 20% (cottonseed meal) was the lowest, but there was no significant difference between four kinds of treatments (P>0.05).Specific radioactivity of free Leucine in plasma complied with significantly exponential function(P<0.05). |
| Key words: free Leucine in plasma special radioactivity young hens |
